                         SEQUENCE LISTING N413422WO

<110>  THE UNIVERSITY OF QUEENSLAND
 
<120>  NEXT-GENERATION ELECTROCHEMICAL BIOSENSORS

<130>  N413422WO

<150>  AU 2018901360
<151>  2018-04-24

<150>  AU 2019900781
<151>  2019-03-08

<160>  86    

<170>  PatentIn version 3.5

<210>  1

<211>  611

<212>  PRT

<213>  Artificial Sequence

<220>
<223>  4th generation GDH-CaM

<400>  1

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 

Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 

Phe Lys Ser Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys 
                405                 410                 415     

Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
            420                 425                 430         

Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
        435                 440                 445             

Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
    450                 455                 460                 

Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
465                 470                 475                 480 

Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
                485                 490                 495     

Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met 
            500                 505                 510         

Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
        515                 520                 525             

Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
    530                 535                 540                 

Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Asn Arg Tyr Arg Asp 
545                 550                 555                 560 


Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr 
                565                 570                 575     

Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu 
            580                 585                 590         

Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys His His His 
        595                 600                 605             

His His His 
    610     


<210>  2
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calmodulin (CaM)

<400>  2

Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp 
1               5                   10                  15      

Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr Val Met 
            20                  25                  30          

Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp Met Ile 
        35                  40                  45              

Asn Glu Val Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe 
    50                  55                  60                  

Leu Thr Met Met Ala Arg Lys Met Lys Asp Thr Asp Ser Glu Glu Glu 
65                  70                  75                  80  

Ile Arg Glu Ala Phe Arg Val Phe Asp Lys Asp Gly Asn Gly Tyr Ile 
                85                  90                  95      

Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu Lys Leu 
            100                 105                 110         

Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile Asp Gly 
        115                 120                 125             

Asp Gly Gln Val Asn Tyr Glu Glu Phe Val Gln Met Met Thr Ala 
    130                 135                 140             

<210>  3
<211>  454
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PQQ-GDH without insert

<400>  3

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 

Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 

Phe Lys Ser Asn Asn Arg Tyr Arg Asp Val Ile Ala Ser Pro Asp Gly 
                405                 410                 415     

Asn Val Leu Tyr Val Leu Thr Asp Thr Ala Gly Asn Val Gln Lys Asp 
            420                 425                 430         

Asp Gly Ser Val Thr Asn Thr Leu Glu Asn Pro Gly Ser Leu Ile Lys 
        435                 440                 445             

Phe Thr Tyr Lys Ala Lys 
    450                 


<210>  4
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 1

<400>  4

Gly Ser Gly Gly 
1               


<210>  5
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 2

<400>  5

Gly Gly Ser Gly Gly 
1               5   


<210>  6
<211>  730
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4th generation Rapamycin and FK506 (Tacrolimus) sensor component 
       2 (GDH-CaM-FKBP)

<400>  6

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 

Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 

Phe Lys Ser Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys 
                405                 410                 415     

Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
            420                 425                 430         

Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
        435                 440                 445             

Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
    450                 455                 460                 

Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
465                 470                 475                 480 

Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
                485                 490                 495     

Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met 
            500                 505                 510         

Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
        515                 520                 525             

Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
    530                 535                 540                 

Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Asn Arg Tyr Arg Asp 
545                 550                 555                 560 

Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr 
                565                 570                 575     

Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu 
            580                 585                 590         

Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys Gly Gly Ser 
        595                 600                 605             

Gly Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr 
    610                 615                 620                 

Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu 
625                 630                 635                 640 

Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe 
                645                 650                 655     

Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly 
            660                 665                 670         

Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro 
        675                 680                 685             

Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His 
    690                 695                 700                 

Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Lys Leu Ala 
705                 710                 715                 720 

Ala Ala Leu Glu His His His His His His 
                725                 730 

<210>  7
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP

<400>  7

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      

Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          

Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              

Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  

Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  

Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      

Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Lys Leu Ala Ala Ala 
            100                 105                 110         

Leu Glu 
        

<210>  8
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  6 x His-tag

<400>  8

His His His His His His 
1               5       


<210>  9
<211>  137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4th and 5th generation Rapamycin sensor component 1 (FRB-BP)

<400>  9

Ala His His His His His His Ser Ser Gly Thr Arg Val Ala Ile Leu 
1               5                   10                  15      

Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr 
            20                  25                  30          

Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu 
        35                  40                  45              

His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe 
    50                  55                  60                  

Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg 
65                  70                  75                  80  

Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Thr Gln Ala Trp Asp 
                85                  90                  95      

Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Gly Ser Gly Gly Ser 
            100                 105                 110         

Gly Ser Gly Ser Gly Gly Ser Gly Gly Lys Arg Arg Trp Lys Lys Asn 
        115                 120                 125             

Phe Ile Ala Val Ser Ala Ala Asn Arg 
    130                 135         


<210>  10
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  higher affinity Calmodulin binding peptide

<400>  10

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


<210>  11
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB

<400>  11

Arg Val Ala Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
1               5                   10                  15      

Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
            20                  25                  30          

Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
        35                  40                  45              

Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
    50                  55                  60                  

Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu 
65                  70                  75                  80  

Thr Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser 
                85                  90                  95  

<210>  12
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 3

<400>  12

Gly Gly Ser Gly Gly Ser Gly Ser Gly Ser Gly Gly Ser Gly Gly 
1               5                   10                  15  


<210>  13
<211>  724
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4th and 5th generation FK506 (Tacrolimus) sensor component 1 
       (SUMO-CN alpha/beta-BP)

<400>  13

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Gly Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro 
            20                  25                  30          

Glu Val Lys Pro Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val 
        35                  40                  45              

Ser Asp Gly Ser Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro 
    50                  55                  60                  

Leu Arg Arg Leu Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met 
65                  70                  75                  80  

Asp Ser Leu Arg Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln 
                85                  90                  95      

Thr Pro Glu Asp Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His 
            100                 105                 110         

Arg Glu Gln Ile Gly Gly Gly Ser Gly Ser Gly Gly Ala His His His 
        115                 120                 125             

His His His Ser Ser Gly Thr Ser Glu Pro Lys Ala Ile Asp Pro Lys 
    130                 135                 140                 

Leu Ser Thr Thr Asp Arg Val Val Lys Ala Val Pro Phe Pro Pro Ser 
145                 150                 155                 160 

His Arg Leu Thr Ala Lys Glu Val Phe Asp Asn Asp Gly Lys Pro Arg 
                165                 170                 175     

Val Asp Ile Leu Lys Ala His Leu Met Lys Glu Gly Arg Leu Glu Glu 
            180                 185                 190         

Ser Val Ala Leu Arg Ile Ile Thr Glu Gly Ala Ser Ile Leu Arg Gln 
        195                 200                 205             

Glu Lys Asn Leu Leu Asp Ile Asp Ala Pro Val Thr Val Cys Gly Asp 
    210                 215                 220                 

Ile His Gly Gln Phe Phe Asp Leu Met Lys Leu Phe Glu Val Gly Gly 
225                 230                 235                 240 

Ser Pro Ala Asn Thr Arg Tyr Leu Phe Leu Gly Asp Tyr Val Asp Arg 
                245                 250                 255     

Gly Tyr Phe Ser Ile Glu Cys Val Leu Tyr Leu Trp Ala Leu Lys Ile 
            260                 265                 270         

Leu Tyr Pro Lys Thr Leu Phe Leu Leu Arg Gly Asn His Glu Cys Arg 
        275                 280                 285             

His Leu Thr Glu Tyr Phe Thr Phe Lys Gln Glu Cys Lys Ile Lys Tyr 
    290                 295                 300                 

Ser Glu Arg Val Tyr Asp Ala Cys Met Asp Ala Phe Asp Cys Leu Pro 
305                 310                 315                 320 

Leu Ala Ala Leu Met Asn Gln Gln Phe Leu Cys Val His Gly Gly Leu 
                325                 330                 335     

Ser Pro Glu Ile Asn Thr Leu Asp Asp Ile Arg Lys Leu Asp Arg Phe 
            340                 345                 350         

Lys Glu Pro Pro Ala Tyr Gly Pro Met Cys Asp Ile Leu Trp Ser Asp 
        355                 360                 365             

Pro Leu Glu Asp Phe Gly Asn Glu Lys Thr Gln Glu His Phe Thr His 
    370                 375                 380                 

Asn Thr Val Arg Gly Cys Ser Tyr Phe Tyr Ser Tyr Pro Ala Val Cys 
385                 390                 395                 400 

Glu Phe Leu Gln His Asn Asn Leu Leu Ser Ile Leu Arg Ala His Glu 
                405                 410                 415     

Ala Gln Asp Ala Gly Tyr Arg Met Tyr Arg Lys Ser Gln Thr Thr Gly 
            420                 425                 430         

Phe Pro Ser Leu Ile Thr Ile Phe Ser Ala Pro Asn Tyr Leu Asp Val 
        435                 440                 445             

Tyr Asn Asn Lys Ala Ala Val Leu Lys Tyr Glu Asn Asn Val Met Asn 
    450                 455                 460                 

Ile Arg Gln Phe Asn Cys Ser Pro His Pro Tyr Trp Leu Pro Asn Phe 
465                 470                 475                 480 

Met Asp Val Phe Thr Trp Ser Leu Pro Phe Val Gly Glu Lys Val Thr 
                485                 490                 495     

Glu Met Leu Val Asn Val Leu Asn Ile Cys Ser Asp Asp Glu Leu Gly 
            500                 505                 510         

Ser Glu Glu Asp Gly Ser Gly Ser Gly Ser Gly Gly Gly Asn Glu Ala 
        515                 520                 525             

Ser Tyr Pro Leu Glu Met Cys Ser His Phe Asp Ala Asp Glu Ile Lys 
    530                 535                 540                 

Arg Leu Gly Lys Arg Phe Lys Lys Leu Asp Leu Asp Asn Ser Gly Ser 
545                 550                 555                 560 

Leu Ser Val Glu Glu Phe Met Ser Leu Pro Glu Leu Gln Gln Asn Pro 
                565                 570                 575     

Leu Val Gln Arg Val Ile Asp Ile Phe Asp Thr Asp Gly Asn Gly Glu 
            580                 585                 590         

Val Asp Phe Lys Glu Phe Ile Glu Gly Val Ser Gln Phe Ser Val Lys 
        595                 600                 605             

Gly Asp Lys Glu Gln Lys Leu Arg Phe Ala Phe Arg Ile Tyr Asp Met 
    610                 615                 620                 

Asp Lys Asp Gly Tyr Ile Ser Asn Gly Glu Leu Phe Gln Val Leu Lys 
625                 630                 635                 640 

Met Met Val Gly Asn Asn Leu Lys Asp Thr Gln Leu Gln Gln Ile Val 
                645                 650                 655     

Asp Lys Thr Ile Ile Asn Ala Asp Lys Asp Gly Asp Gly Arg Ile Ser 
            660                 665                 670         

Phe Glu Glu Phe Cys Ala Val Val Gly Gly Leu Asp Ile His Lys Lys 
        675                 680                 685             

Met Val Val Asp Val Gly Gly Ser Gly Gly Ser Gly Ser Gly Ser Gly 
    690                 695                 700                 

Gly Ser Gly Gly Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser 
705                 710                 715                 720 

Ala Ala Asn Arg 
                

<210>  14
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SUMO

<400>  14

Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn Phe Asp Lys Lys 
1               5                   10                  15      

Gly Ser Asp Ser Glu Val Asn Gln Glu Ala Lys Pro Glu Val Lys Pro 
            20                  25                  30          

Glu Val Lys Pro Glu Thr His Ile Asn Leu Lys Val Ser Asp Gly Ser 
        35                  40                  45              

Ser Glu Ile Phe Phe Lys Ile Lys Lys Thr Thr Pro Leu Arg Arg Leu 
    50                  55                  60                  

Met Glu Ala Phe Ala Lys Arg Gln Gly Lys Glu Met Asp Ser Leu Arg 
65                  70                  75                  80  

Phe Leu Tyr Asp Gly Ile Arg Ile Gln Ala Asp Gln Thr Pro Glu Asp 
                85                  90                  95      

Leu Asp Met Glu Asp Asn Asp Ile Ile Glu Ala His Arg Glu Gln Ile 
            100                 105                 110         

Gly Gly 
        

<210>  15
<211>  381
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calcinurin alpha subunit (CN alpha)

<400>  15

Ser Glu Pro Lys Ala Ile Asp Pro Lys Leu Ser Thr Thr Asp Arg Val 
1               5                   10                  15      

Val Lys Ala Val Pro Phe Pro Pro Ser His Arg Leu Thr Ala Lys Glu 
            20                  25                  30          

Val Phe Asp Asn Asp Gly Lys Pro Arg Val Asp Ile Leu Lys Ala His 
        35                  40                  45              

Leu Met Lys Glu Gly Arg Leu Glu Glu Ser Val Ala Leu Arg Ile Ile 
    50                  55                  60                  

Thr Glu Gly Ala Ser Ile Leu Arg Gln Glu Lys Asn Leu Leu Asp Ile 
65                  70                  75                  80  

Asp Ala Pro Val Thr Val Cys Gly Asp Ile His Gly Gln Phe Phe Asp 
                85                  90                  95      

Leu Met Lys Leu Phe Glu Val Gly Gly Ser Pro Ala Asn Thr Arg Tyr 
            100                 105                 110         

Leu Phe Leu Gly Asp Tyr Val Asp Arg Gly Tyr Phe Ser Ile Glu Cys 
        115                 120                 125             

Val Leu Tyr Leu Trp Ala Leu Lys Ile Leu Tyr Pro Lys Thr Leu Phe 
    130                 135                 140                 

Leu Leu Arg Gly Asn His Glu Cys Arg His Leu Thr Glu Tyr Phe Thr 
145                 150                 155                 160 

Phe Lys Gln Glu Cys Lys Ile Lys Tyr Ser Glu Arg Val Tyr Asp Ala 
                165                 170                 175     

Cys Met Asp Ala Phe Asp Cys Leu Pro Leu Ala Ala Leu Met Asn Gln 
            180                 185                 190         

Gln Phe Leu Cys Val His Gly Gly Leu Ser Pro Glu Ile Asn Thr Leu 
        195                 200                 205             

Asp Asp Ile Arg Lys Leu Asp Arg Phe Lys Glu Pro Pro Ala Tyr Gly 
    210                 215                 220                 

Pro Met Cys Asp Ile Leu Trp Ser Asp Pro Leu Glu Asp Phe Gly Asn 
225                 230                 235                 240 

Glu Lys Thr Gln Glu His Phe Thr His Asn Thr Val Arg Gly Cys Ser 
                245                 250                 255     

Tyr Phe Tyr Ser Tyr Pro Ala Val Cys Glu Phe Leu Gln His Asn Asn 
            260                 265                 270         

Leu Leu Ser Ile Leu Arg Ala His Glu Ala Gln Asp Ala Gly Tyr Arg 
        275                 280                 285             

Met Tyr Arg Lys Ser Gln Thr Thr Gly Phe Pro Ser Leu Ile Thr Ile 
    290                 295                 300                 

Phe Ser Ala Pro Asn Tyr Leu Asp Val Tyr Asn Asn Lys Ala Ala Val 
305                 310                 315                 320 

Leu Lys Tyr Glu Asn Asn Val Met Asn Ile Arg Gln Phe Asn Cys Ser 
                325                 330                 335     

Pro His Pro Tyr Trp Leu Pro Asn Phe Met Asp Val Phe Thr Trp Ser 
            340                 345                 350         

Leu Pro Phe Val Gly Glu Lys Val Thr Glu Met Leu Val Asn Val Leu 
        355                 360                 365             

Asn Ile Cys Ser Asp Asp Glu Leu Gly Ser Glu Glu Asp 
    370                 375                 380     

<210>  16
<211>  168
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calcinurin beta subunit (CN beta)

<400>  16

Asn Glu Ala Ser Tyr Pro Leu Glu Met Cys Ser His Phe Asp Ala Asp 
1               5                   10                  15      

Glu Ile Lys Arg Leu Gly Lys Arg Phe Lys Lys Leu Asp Leu Asp Asn 
            20                  25                  30          

Ser Gly Ser Leu Ser Val Glu Glu Phe Met Ser Leu Pro Glu Leu Gln 
        35                  40                  45              

Gln Asn Pro Leu Val Gln Arg Val Ile Asp Ile Phe Asp Thr Asp Gly 
    50                  55                  60                  

Asn Gly Glu Val Asp Phe Lys Glu Phe Ile Glu Gly Val Ser Gln Phe 
65                  70                  75                  80  

Ser Val Lys Gly Asp Lys Glu Gln Lys Leu Arg Phe Ala Phe Arg Ile 
                85                  90                  95      

Tyr Asp Met Asp Lys Asp Gly Tyr Ile Ser Asn Gly Glu Leu Phe Gln 
            100                 105                 110         

Val Leu Lys Met Met Val Gly Asn Asn Leu Lys Asp Thr Gln Leu Gln 
        115                 120                 125             

Gln Ile Val Asp Lys Thr Ile Ile Asn Ala Asp Lys Asp Gly Asp Gly 
    130                 135                 140                 

Arg Ile Ser Phe Glu Glu Phe Cys Ala Val Val Gly Gly Leu Asp Ile 
145                 150                 155                 160 

His Lys Lys Met Val Val Asp Val 
                165             

<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 4

<400>  17

Gly Gly Gly Ser Gly Ser Gly Gly 
1               5               


<210>  18
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 5

<400>  18

Gly Ser Gly Ser Gly Ser Gly Gly Gly 
1               5                   


<210>  19
<211>  698
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4th generation Amylase sensor component 2 (GDH-CaM-VHH1)

<400>  19

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 

Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 

Phe Lys Ser Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys 
                405                 410                 415     

Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
            420                 425                 430         

Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
        435                 440                 445             

Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
    450                 455                 460                 

Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
465                 470                 475                 480 

Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
                485                 490                 495     

Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met 
            500                 505                 510         

Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
        515                 520                 525             

Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
    530                 535                 540                 

Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Asn Arg Tyr Arg Asp 
545                 550                 555                 560 

Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr 
                565                 570                 575     

Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu 
            580                 585                 590         

Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys Gly Ser Gly 
        595                 600                 605             

Ser Gly Gly Asp Thr Thr Val Ser Glu Pro Ala Pro Ser Cys Val Thr 
    610                 615                 620                 

Leu Tyr Gln Ser Trp Arg Tyr Ser Gln Ala Asp Asn Gly Cys Ala Glu 
625                 630                 635                 640 

Thr Val Thr Val Lys Val Val Tyr Glu Asp Asp Thr Glu Gly Leu Cys 
                645                 650                 655     

Tyr Ala Val Ala Pro Gly Gln Ile Thr Thr Val Gly Asp Gly Tyr Ile 
            660                 665                 670         

Gly Ser His Gly His Ala Arg Tyr Leu Ala Arg Cys Leu Lys Leu Ala 
        675                 680                 685             

Ala Ala Leu Glu His His His His His His 
    690                 695             

<210>  20
<211>  74
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH1

<400>  20

Asp Thr Thr Val Ser Glu Pro Ala Pro Ser Cys Val Thr Leu Tyr Gln 
1               5                   10                  15      

Ser Trp Arg Tyr Ser Gln Ala Asp Asn Gly Cys Ala Glu Thr Val Thr 
            20                  25                  30          

Val Lys Val Val Tyr Glu Asp Asp Thr Glu Gly Leu Cys Tyr Ala Val 
        35                  40                  45              

Ala Pro Gly Gln Ile Thr Thr Val Gly Asp Gly Tyr Ile Gly Ser His 
    50                  55                  60                  

Gly His Ala Arg Tyr Leu Ala Arg Cys Leu 
65                  70                  


<210>  21
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4th and 5th generation Amylase sensor component 1: VHH2-BP

<400>  21

Asp Gly His His His His His His Gly Ser Gly Gln Val Gln Leu Val 
1               5                   10                  15      

Glu Ser Gly Gly Gly Thr Val Pro Ala Gly Gly Ser Leu Arg Leu Ser 
            20                  25                  30          

Cys Ala Ala Ser Gly Asn Thr Leu Cys Thr Tyr Asp Met Ser Trp Tyr 
        35                  40                  45              

Arg Arg Ala Pro Gly Lys Gly Arg Asp Phe Val Ser Gly Ile Asp Asn 
    50                  55                  60                  

Asp Gly Thr Thr Thr Tyr Val Asp Ser Val Ala Gly Arg Phe Thr Ile 
65                  70                  75                  80  

Ser Gln Gly Asn Ala Lys Asn Thr Ala Tyr Leu Gln Met Asp Ser Leu 
                85                  90                  95      

Lys Pro Asp Asp Thr Ala Met Tyr Tyr Cys Lys Pro Ser Leu Arg Tyr 
            100                 105                 110         

Gly Leu Pro Gly Cys Pro Ile Ile Pro Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             

Thr Val Ser Ser Gly Gly Ser Gly Gly Ser Gly Ser Gly Ser Gly Gly 
    130                 135                 140                 

Ser Gly Gly Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala 
145                 150                 155                 160 

Ala Asn Arg 
            

<210>  22
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH2

<400>  22

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Thr Val Pro Ala Gly Gly 
1               5                   10                  15      

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Asn Thr Leu Cys Thr Tyr 
            20                  25                  30          

Asp Met Ser Trp Tyr Arg Arg Ala Pro Gly Lys Gly Arg Asp Phe Val 
        35                  40                  45              

Ser Gly Ile Asp Asn Asp Gly Thr Thr Thr Tyr Val Asp Ser Val Ala 
    50                  55                  60                  

Gly Arg Phe Thr Ile Ser Gln Gly Asn Ala Lys Asn Thr Ala Tyr Leu 
65                  70                  75                  80  

Gln Met Asp Ser Leu Lys Pro Asp Asp Thr Ala Met Tyr Tyr Cys Lys 
                85                  90                  95      

Pro Ser Leu Arg Tyr Gly Leu Pro Gly Cys Pro Ile Ile Pro Trp Gly 
            100                 105                 110         

Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     

<210>  23
<211>  618
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5th generation GDH-CaM

<400>  23

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 

Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 

Phe Lys Ser Gly Cys Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys 
                405                 410                 415     

Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
            420                 425                 430         

Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
        435                 440                 445             

Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
    450                 455                 460                 

Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
465                 470                 475                 480 

Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
                485                 490                 495     

Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met 
            500                 505                 510         

Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
        515                 520                 525             

Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
    530                 535                 540                 

Val Gln Met Met Thr Ala Gly Gly Ser Cys Gly Asn Arg Tyr Arg Asp 
545                 550                 555                 560 

Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr 
                565                 570                 575     

Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu 
            580                 585                 590         

Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys Lys Leu Ala 
        595                 600                 605             

Ala Ala Leu Glu His His His His His His 
    610                 615             


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 6

<400>  24

Gly Cys Gly Gly 
1               


<210>  25
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker 7

<400>  25

Gly Gly Ser Cys Gly 
1               5   


<210>  26
<211>  730
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5th generation Rapamycin and FK506 sensor component 2 
       (GDH-CaM-FKBP)

<400>  26

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 

Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 

Phe Lys Ser Gly Cys Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys 
                405                 410                 415     

Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
            420                 425                 430         

Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
        435                 440                 445             

Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
    450                 455                 460                 

Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
465                 470                 475                 480 

Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
                485                 490                 495     

Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met 
            500                 505                 510         

Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
        515                 520                 525             

Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
    530                 535                 540                 

Val Gln Met Met Thr Ala Gly Gly Ser Cys Gly Asn Arg Tyr Arg Asp 
545                 550                 555                 560 

Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr 
                565                 570                 575     

Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu 
            580                 585                 590         

Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys Gly Gly Ser 
        595                 600                 605             

Gly Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr 
    610                 615                 620                 

Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu 
625                 630                 635                 640 

Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe 
                645                 650                 655     

Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly 
            660                 665                 670         

Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro 
        675                 680                 685             

Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His 
    690                 695                 700                 

Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Lys Leu Ala 
705                 710                 715                 720 

Ala Ala Leu Glu His His His His His His 
                725                 730 

<210>  27
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calmodulin having reduced affinity for CaM-BP (CaM*1)

<400>  27

Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp 
1               5                   10                  15      

Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr Val Met 
            20                  25                  30          

Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp Met Ile 
        35                  40                  45              

Asn Glu Val Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe 
    50                  55                  60                  

Leu Thr Met Met Ala Arg Lys Met Lys Asp Thr Asp Ser Glu Glu Glu 
65                  70                  75                  80  

Ile Arg Glu Ala Phe Arg Val Ala Asp Lys Asp Gly Asn Gly Tyr Ile 
                85                  90                  95      

Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu Lys Leu 
            100                 105                 110         

Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile Asp Gly 
        115                 120                 125             

Asp Gly Gln Val Asn Tyr Glu Glu Ala Val Gln Met Met Thr Ala 
    130                 135                 140             


<210>  28
<211>  181
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  caged CaM-BP - CaM*1 fusion

<400>  28

Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp 
1               5                   10                  15      

Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr Val Met 
            20                  25                  30          

Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp Met Ile 
        35                  40                  45              

Asn Glu Val Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe 
    50                  55                  60                  

Leu Thr Met Met Ala Arg Lys Met Lys Asp Thr Asp Ser Glu Glu Glu 
65                  70                  75                  80  

Ile Arg Glu Ala Phe Arg Val Ala Asp Lys Asp Gly Asn Gly Tyr Ile 
                85                  90                  95      

Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu Lys Leu 
            100                 105                 110         

Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile Asp Gly 
        115                 120                 125             

Asp Gly Gln Val Asn Tyr Glu Glu Ala Val Gln Met Met Thr Ala Gly 
    130                 135                 140                 

Gly Ser Gly Gly Ser Gly Ser Gly Ser Gly Gly Lys Arg Arg Trp Lys 
145                 150                 155                 160 

Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg Phe Lys Lys Ile Ser 
                165                 170                 175     

Ser Ser Gly Ala Leu 
            180     


<210>  29
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wild type CaM-BP

<400>  29

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      

Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  30
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB-CaM*1-CaMBP fusion

<400>  30

Met Ala His His His His His His Ser Ser Gly Thr Arg Val Ala Ile 
1               5                   10                  15      

Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu 
            20                  25                  30          

Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro 
        35                  40                  45              

Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser 
    50                  55                  60                  

Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys 
65                  70                  75                  80  

Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Thr Gln Ala Trp 
                85                  90                  95      

Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Gly Ser Gly Gly 
            100                 105                 110         

Ser Gly Ser Gly Ser Gly Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala 
        115                 120                 125             

Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr 
    130                 135                 140                 

Ile Thr Thr Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn 
145                 150                 155                 160 

Pro Thr Glu Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp 
                165                 170                 175     

Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg 
            180                 185                 190         

Lys Met Lys Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg 
        195                 200                 205             

Val Ala Asp Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg 
    210                 215                 220                 

His Val Met Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp 
225                 230                 235                 240 

Glu Met Ile Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr 
                245                 250                 255     

Glu Glu Ala Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Ser Gly 
            260                 265                 270         

Ser Gly Ser Gly Gly Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val 
        275                 280                 285             

Ser Ala Ala Asn Arg Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
    290                 295                 300             


<210>  31
<211>  382
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-TVMV-AI including thrombin cleavage site

<400>  31

Met Asp Gly Ser Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly 
1               5                   10                  15      

Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly 
            20                  25                  30          

Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys 
        35                  40                  45             

Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu 
    50                  55                  60                  

Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile 
65                  70                  75                  80  

Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro 
                85                  90                  95      

Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly 
            100                 105                 110         

Gly Ser Gly Gly Gly Ser Gly Ser Gly Gly Ser Gly Gly Ser Ser Lys 
        115                 120                 125             

Ala Leu Leu Lys Gly Val Arg Asp Phe Asn Pro Ile Ser Ala Cys Val 
    130                 135                 140                 

Cys Leu Leu Glu Asn Ser Ser Asp Gly His Ser Glu Arg Leu Phe Gly 
145                 150                 155                 160 

Ile Gly Phe Gly Pro Tyr Ile Ile Ala Asn Gln His Leu Phe Arg Arg 
                165                 170                 175     

Asn Asn Gly Glu Leu Thr Ile Lys Thr Met His Gly Glu Phe Lys Val 
            180                 185                 190         

Lys Asn Ser Thr Gln Leu Gln Met Lys Pro Val Glu Gly Arg Asp Ile 
        195                 200                 205             

Ile Val Ile Lys Met Ala Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu 
    210                 215                 220                 

Lys Phe Arg Gln Pro Thr Ile Lys Asp Arg Val Cys Met Val Ser Thr 
225                 230                 235                 240 

Asn Phe Gln Gln Lys Ser Val Ser Ser Leu Val Ser Glu Ser Ser His 
                245                 250                 255     

Ile Val His Lys Glu Asp Thr Ser Phe Trp Gln His Trp Ile Thr Thr 
            260                 265                 270         

Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Ile Ile Asp Gly Asn 
        275                 280                 285             

Ile Leu Gly Ile His Ser Leu Thr His Thr Thr Asn Gly Ser Asn Tyr 
    290                 295                 300                 

Phe Val Glu Phe Pro Glu Lys Phe Val Ala Thr Tyr Leu Asp Ala Ala 
305                 310                 315                 320 

Asp Gly Trp Cys Lys Asn Trp Lys Phe Asn Ala Asp Lys Ile Ser Trp 
                325                 330                 335     

Gly Ser Phe Ile Leu Trp Glu Asp Ala Pro Glu Asp Phe Met Ser Gly 
            340                 345                 350         

Leu Val Pro Arg Gly Val Gly Arg Glu Tyr Val Arg Phe Ala Pro Gly 
        355                 360                 365             

Ser Lys Leu Ala Ala Ala Leu Glu His His His His His His 
    370                 375                 380         


<210>  32
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TVMV

<400>  32

Ser Ser Lys Ala Leu Leu Lys Gly Val Arg Asp Phe Asn Pro Ile Ser 
1               5                   10                  15      

Ala Cys Val Cys Leu Leu Glu Asn Ser Ser Asp Gly His Ser Glu Arg 
            20                  25                  30          

Leu Phe Gly Ile Gly Phe Gly Pro Tyr Ile Ile Ala Asn Gln His Leu 
        35                  40                  45              

Phe Arg Arg Asn Asn Gly Glu Leu Thr Ile Lys Thr Met His Gly Glu 
    50                  55                  60                  

Phe Lys Val Lys Asn Ser Thr Gln Leu Gln Met Lys Pro Val Glu Gly 
65                  70                  75                  80  

Arg Asp Ile Ile Val Ile Lys Met Ala Lys Asp Phe Pro Pro Phe Pro 
                85                  90                  95      

Gln Lys Leu Lys Phe Arg Gln Pro Thr Ile Lys Asp Arg Val Cys Met 
            100                 105                 110         

Val Ser Thr Asn Phe Gln Gln Lys Ser Val Ser Ser Leu Val Ser Glu 
        115                 120                 125             

Ser Ser His Ile Val His Lys Glu Asp Thr Ser Phe Trp Gln His Trp 
    130                 135                 140                 

Ile Thr Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Ile Ile 
145                 150                 155                 160 

Asp Gly Asn Ile Leu Gly Ile His Ser Leu Thr His Thr Thr Asn Gly 
                165                 170                 175     

Ser Asn Tyr Phe Val Glu Phe Pro Glu Lys Phe Val Ala Thr Tyr Leu 
            180                 185                 190         

Asp Ala Ala Asp Gly Trp Cys Lys Asn Trp Lys Phe Asn Ala Asp Lys 
        195                 200                 205             

Ile Ser Trp Gly Ser Phe Ile Leu Trp Glu Asp Ala Pro Glu Asp Phe 
    210                 215                 220                 


Met Ser 
225     


<210>  33
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  autoinhibitory peptide (AI)

<400>  33

Arg Glu Tyr Val Arg Phe Ala Pro 
1               5               


<210>  34
<211>  323
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB-CaM*1-TVMV cleavage site-CaM-BP

<400>  34

Met Ala His His His His His His Ser Ser Gly Thr Arg Val Ala Ile 
1               5                   10                  15      

Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu 
            20                  25                  30          

Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro 
        35                  40                  45              

Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser 
    50                  55                  60                  

Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys 
65                  70                  75                  80  

Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Thr Gln Ala Trp 
                85                  90                  95      

Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Gly Ser Gly Gly 
            100                 105                 110         

Ser Gly Ser Gly Ser Gly Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala 
        115                 120                 125             

Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr 
    130                 135                 140                 

Ile Thr Thr Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn 
145                 150                 155                 160 

Pro Thr Glu Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp 
                165                 170                 175     

Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg 
            180                 185                 190         

Lys Met Lys Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg 
        195                 200                 205             

Val Ala Asp Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg 
    210                 215                 220                 

His Val Met Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp 
225                 230                 235                 240 

Glu Met Ile Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr 
                245                 250                 255     

Glu Glu Ala Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Ser Glu 
            260                 265                 270         

Thr Val Arg Phe Gln Ser Gly Gly Ser Asp Asp Val Thr Pro Cys Ser 
        275                 280                 285             

Met Ser Leu Gly Ser Gly Ser Gly Gly Lys Arg Arg Trp Lys Lys Asn 
    290                 295                 300                 

Phe Ile Ala Val Ser Ala Ala Asn Arg Phe Lys Lys Ile Ser Ser Ser 
305                 310                 315                 320 


Gly Ala Leu 
            

<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TVMV cleavage site

<400>  35

Glu Thr Val Arg Phe Gln Ser 
1               5           


<210>  36
<211>  613
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Q2GDH-CaM (mutant of 3rd generation GDH)

<400>  36

Met Gly Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser 
1               5                   10                  15      

Glu Asn Phe Asp Lys Gln Val Ile Leu Ser Asn Leu Asn Lys Pro His 
            20                  25                  30          

Ala Leu Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala 
        35                  40                  45              

Thr Gly Gln Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr 
    50                  55                  60                  

Val Phe Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly 
65                  70                  75                  80  

Leu Leu Gly Phe Ala Phe His Pro Asp Phe Gln Asn Asn Pro Tyr Ile 
                85                  90                  95      

Tyr Ile Ser Gly Thr Phe Gln Asn Pro Gln Ser Thr Asp Gln Glu Leu 
            100                 105                 110         

Pro Asn Gln Thr Ile Ile Arg Gln Tyr Thr Tyr Asn Gln Ser Thr Asp 
        115                 120                 125             

Thr Leu Glu Gln Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys 
    130                 135                 140                 

Asp His Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr 
145                 150                 155                 160 

Tyr Thr Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu 
                165                 170                 175     

Pro Asn Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Gln 
            180                 185                 190         

Asp Tyr His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly 
        195                 200                 205             

Ser Ile Pro Gln Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile 
    210                 215                 220                 

Tyr Thr Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn 
225                 230                 235                 240 

Gly Lys Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile 
                245                 250                 255     

Asn Leu Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly 
            260                 265                 270         

Tyr Lys Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala 
        275                 280                 285             

Asn Lys Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala 
    290                 295                 300                 

Gly Val Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val 
305                 310                 315                 320 

Pro Pro Leu Lys Thr Leu Tyr Thr Val Gln Asp Gly Ser Gly Gly Thr 
                325                 330                 335     

Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp Lys 
            340                 345                 350         

Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr Val Met Arg 
        355                 360                 365             

Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp Met Ile Asn 
    370                 375                 380                 

Glu Val Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe Leu 
385                 390                 395                 400 

Thr Met Met Ala Arg Lys Met Lys Asp Thr Asp Ser Glu Glu Glu Ile 
                405                 410                 415     

Arg Glu Ala Phe Arg Val Phe Asp Lys Asp Gly Asn Gly Tyr Ile Ser 
            420                 425                 430         

Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu Lys Leu Thr 
        435                 440                 445             

Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile Asp Gly Asp 
    450                 455                 460                 

Gly Gln Val Asn Tyr Glu Glu Phe Val Gln Met Met Thr Ala Gly Gly 
465                 470                 475                 480 

Ser Gly Gly Tyr Asn Tyr Asn Asp Pro Thr Cys Gly Glu Met Thr Tyr 
                485                 490                 495     

Ile Cys Trp Pro Thr Val Ala Pro Ser Ser Ala Tyr Val Tyr Gln Gly 
            500                 505                 510         

Gly Gln Gln Ala Ile Thr Gly Trp Glu Asn Thr Leu Leu Val Pro Ser 
        515                 520                 525             

Leu Lys Arg Gly Val Ile Phe Arg Ile Lys Leu Asp Pro Thr Tyr Ser 
    530                 535                 540                 


Thr Thr Tyr Asp Asp Ala Val Pro Met Phe Lys Ser Asn Asn Arg Tyr 
545                 550                 555                 560 

Arg Asp Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr 
                565                 570                 575     

Asp Thr Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr 
            580                 585                 590         

Leu Glu Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys His 
        595                 600                 605             

His His His His His 
    610             

<210>  37
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  lower affinity CaM-BP

<400>  37

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ala Ser Ala Ser Ala 
1               5                   10                  15      


<210>  38
<211>  403
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal PQQ-GDH fragment

<400>  38

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 


Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 


Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 


Phe Lys Ser 
            

<210>  39
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal PQQ-GDH fragment

<400>  39

Asn Arg Tyr Arg Asp Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr 
1               5                   10                  15      

Val Leu Thr Asp Thr Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val 
            20                  25                  30          

Thr Asn Thr Leu Glu Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys 
        35                  40                  45              

Ala Lys
    50  


<210>  40
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker in caged CaM-BP - CaM*1 fusion

<400>  40

Gly Gly Ser Gly Gly Ser Gly Ser Gly Ser Gly Gly 
1               5                   10          


<210>  41
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP-TVMV linker

<400>  41

Gly Gly Ser Gly Gly Gly Ser Gly Ser Gly Gly Ser Gly Gly Ser Ser 
1               5                   10                  15      

<210>  42
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TVMV-AI

<400>  42

Ser Ser Lys Ala Leu Leu Lys Gly Val Arg Asp Phe Asn Pro Ile Ser 
1               5                   10                  15      

Ala Cys Val Cys Leu Leu Glu Asn Ser Ser Asp Gly His Ser Glu Arg 
            20                  25                  30          

Leu Phe Gly Ile Gly Phe Gly Pro Tyr Ile Ile Ala Asn Gln His Leu 
        35                  40                  45              

Phe Arg Arg Asn Asn Gly Glu Leu Thr Ile Lys Thr Met His Gly Glu 
    50                  55                  60                  

Phe Lys Val Lys Asn Ser Thr Gln Leu Gln Met Lys Pro Val Glu Gly 
65                  70                  75                  80  

Arg Asp Ile Ile Val Ile Lys Met Ala Lys Asp Phe Pro Pro Phe Pro 
                85                  90                  95      

Gln Lys Leu Lys Phe Arg Gln Pro Thr Ile Lys Asp Arg Val Cys Met 
            100                 105                 110         

Val Ser Thr Asn Phe Gln Gln Lys Ser Val Ser Ser Leu Val Ser Glu 
        115                 120                 125             

Ser Ser His Ile Val His Lys Glu Asp Thr Ser Phe Trp Gln His Trp 
    130                 135                 140                 

Ile Thr Thr Lys Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Ile Ile 
145                 150                 155                 160 

Asp Gly Asn Ile Leu Gly Ile His Ser Leu Thr His Thr Thr Asn Gly 
                165                 170                 175     

Ser Asn Tyr Phe Val Glu Phe Pro Glu Lys Phe Val Ala Thr Tyr Leu 
            180                 185                 190         

Asp Ala Ala Asp Gly Trp Cys Lys Asn Trp Lys Phe Asn Ala Asp Lys 
        195                 200                 205             

Ile Ser Trp Gly Ser Phe Ile Leu Trp Glu Asp Ala Pro Glu Asp Phe 
    210                 215                 220                 

Met Ser Gly Leu Val Pro Arg Gly Val Gly Arg Glu Tyr Val Arg Phe 
225                 230                 235                 240 

Ala Pro Gly Ser Lys Leu Ala Ala Ala Leu Glu His His His His His 
                245                 250                 255     

His 
    

<210>  43
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TVMV cleavage site containing linker in CaM*1-TVMV cleavage 
       site-CaM-BP construct

<400>  43

Gly Gly Ser Gly Gly Ser Glu Thr Val Arg Phe Gln Ser Gly Gly Ser 
1               5                   10                  15      

Asp Asp Val Thr Pro Cys Ser Met Ser Leu Gly Ser Gly Ser Gly Gly 
            20                  25                  30          

<210>  44
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CaM*1-TVMV cleavage site-CaM-BP construct

<400>  44

Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp 
1               5                   10                  15      

Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr Val Met 
            20                  25                  30          

Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp Met Ile 
        35                  40                  45              

Asn Glu Val Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe 
    50                  55                  60                  

Leu Thr Met Met Ala Arg Lys Met Lys Asp Thr Asp Ser Glu Glu Glu 
65                  70                  75                  80  

Ile Arg Glu Ala Phe Arg Val Ala Asp Lys Asp Gly Asn Gly Tyr Ile 
                85                  90                  95     

Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu Lys Leu 
            100                 105                 110         

Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile Asp Gly 
        115                 120                 125             

Asp Gly Gln Val Asn Tyr Glu Glu Ala Val Gln Met Met Thr Ala Gly 
    130                 135                 140                 

Gly Ser Gly Gly Ser Glu Thr Val Arg Phe Gln Ser Gly Gly Ser Asp 
145                 150                 155                 160 

Asp Val Thr Pro Cys Ser Met Ser Leu Gly Ser Gly Ser Gly Gly Lys 
                165                 170                 175     

Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg Phe 
            180                 185                 190         

Lys Lys Ile Ser Ser Ser Gly Ala Leu 
        195                 200     

<210>  45
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 1

<400>  45

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      

Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      

<210>  46
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 1t

<400>  46

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ala Ser Ala Ser Ala 
1               5                   10                  15      


<210>  47
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 2

<400>  47

Glu Val Thr Val Gly Lys Phe Tyr Ala Thr Phe Leu Ile Gln Glu Tyr 
1               5                   10                  15      

Phe Arg Lys Phe Lys Lys Arg Lys Glu Gln Gly Leu Val Gly Lys Pro 
            20                  25                  30          

Ser 
    

<210>  48
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 3

<400>  48

Glu Val Thr Val Gly Lys Phe Tyr Ala Thr Phe Leu Ile Gln 
1               5                   10                  


<210>  49
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 4

<400>  49

His Met Gly Lys Ile Tyr Ala Ala Met Met Ile Met Glu Tyr Tyr Arg 
1               5                   10                  15      

Gln Ser Lys Ala Lys Lys 
            20          

<210>  50
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 5

<400>  50

His Met Gly Lys Ile Tyr Ala Ala Met Met Ile Met Glu Ala Tyr Arg 
1               5                   10                  15      

Gln Ser Lys Ala Lys Lys 
            20          

<210>  51
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 6

<400>  51

Glu Met Arg Gln Lys Ile Arg Ser His Ala His Ala Leu Leu Ala Ala 
1               5                   10                  15      


Asn Arg Phe Met Asp Met 
            20          

<210>  52
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 7

<400>  52

Asn Met Gly Gln His Leu Asp Val Lys Leu Val Pro Ser Ser Ser Tyr 
1               5                   10                  15      

Ile Lys Val Val Lys Ala Phe His His Ser Ser Leu His Glu 
            20                  25                  30  

<210>  53
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 8

<400>  53

Gly Arg Asn Trp Lys Asn Phe Ala Leu Val Pro Leu Leu Arg Asp 
1               5                   10                  15  


<210>  54
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 9

<400>  54

Leu Thr Glu Ile Lys Lys Gln Ile Pro Gln Lys Glu Trp Asp Lys Val 
1               5                   10                  15      

Val Asn Thr Pro Asn Ser Leu Glu Lys Gln Lys Gly Val Thr Asn Leu 
            20                  25                  30          

Leu Ile Lys Tyr Gly Ile Glu Arg 
        35                  40  

<210>  55
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 10

<400>  55

Ile Arg Asn Lys Ile Arg Ala Ile Gly Lys Met Ala Arg Val Phe 
1               5                   10                  15  

<210>  56
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 11

<400>  56

His Ser Met Gln Ala Leu Ser Trp Arg Lys Leu Tyr Leu Ser Arg Ala 
1               5                   10                  15      

Lys Leu Lys Ala 
            20  

<210>  57
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 12

<400>  57

Ala Asp Lys Leu Arg Ala Ala Cys Ile Arg Ile Gln Lys Thr Ile Arg 
1               5                   10                  15      

Gly Trp Leu Leu Arg Lys 
            20          

<210>  58
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 13

<400>  58

Met Gln Arg Ala Ala Ile Thr Val Gln Arg Tyr Val Arg Gly Tyr Gln 
1               5                   10                  15      

Ala Arg Cys Tyr Ala Lys Phe Leu Arg Arg Thr Lys 
            20                  25              

<210>  59
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 14

<400>  59

Glu Ile Arg Lys Ile Leu Arg Asn Asn Leu Gln Lys Thr Arg Gln Arg 
1               5                   10                  15      

Leu Arg Ser Tyr Asn Arg His Thr Leu Val Ala Asp Pro Tyr Glu Glu 
            20                  25                  30          

Ala Trp Asn Gln Met Leu Leu Arg Arg Gln Lys 
        35                  40              

<210>  60
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variant CaM-BP 15

<400>  60

Arg Leu Ser Phe Lys Thr Val Ala Leu Leu Val Leu Ala Cys Val Arg 
1               5                   10                  15      

Met Lys Arg Ile Ala Phe Tyr 
            20              

<210>  61
<211>  303
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB-CaM*2-CaMBP fusion

<400>  61

Met Ala His His His His His His Ser Ser Gly Thr Arg Val Ala Ile 
1               5                   10                  15      

Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu 
            20                  25                  30          

Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro 
        35                  40                  45              

Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser 
    50                  55                  60                  

Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys 
65                  70                  75                  80  

Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Thr Gln Ala Trp 
                85                  90                  95      

Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Gly Ser Gly Gly 
            100                 105                 110         

Ser Gly Ser Gly Ser Gly Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala 
        115                 120                 125             

Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr 
    130                 135                 140                 

Ile Thr Thr Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn 
145                 150                 155                 160 

Pro Thr Glu Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp 
                165                 170                 175     

Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg 
            180                 185                 190         

Lys Met Lys Asp Thr Asp Ser Glu Ser Glu Ile Arg Glu Ala Phe Arg 
        195                 200                 205             

Val Ala Asp Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Ala Arg 
    210                 215                 220                 

His Val Met Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp 
225                 230                 235                 240 

Glu Met Ile Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr 
                245                 250                 255     

Glu Glu Ala Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Ser Gly 
            260                 265                 270         

Ser Gly Ser Gly Gly Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val 
        275                 280                 285             

Ser Ala Ala Asn Arg Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
    290                 295                 300             


<210>  62
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CaM*2

<400>  62

Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp 
1               5                   10                  15      

Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr Val Met 
            20                  25                  30          

Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp Met Ile 
        35                  40                  45              

Asn Glu Val Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe 
    50                  55                  60                  

Leu Thr Met Met Ala Arg Lys Met Lys Asp Thr Asp Ser Glu Ser Glu 
65                  70                  75                  80  

Ile Arg Glu Ala Phe Arg Val Ala Asp Lys Asp Gly Asn Gly Tyr Ile 
                85                  90                  95      

Ser Ala Ala Glu Ala Arg His Val Met Thr Asn Leu Gly Glu Lys Leu 
            100                 105                 110         

Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile Asp Gly 
        115                 120                 125             

Asp Gly Gln Val Asn Tyr Glu Glu Ala Val Gln Met Met Thr Ala 
    130                 135                 140             

<210>  63
<211>  694
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5th generation human serum albumin (HAS) sensor component 1 (GA 
       binder-GDH-CalM)

<400>  63

Ala Val Asp Ala Asn Ser Leu Ala Glu Ala Lys Val Leu Ala Asn Arg 
1               5                   10                  15      

Glu Leu Asp Lys Tyr Gly Val Ser Asp Phe Tyr Lys Arg Leu Ile Asn 
            20                  25                  30          

Lys Ala Lys Thr Val Glu Gly Val Glu Ala Leu Lys Leu His Ile Leu 
        35                  40                  45              

Ala Ala Leu Pro Gly Gly Ser Gly Ser Gly Gly Ser Gly Gly Gly Ser 
    50                  55                  60                  

Gly Ser Ser Gly Gly Ser Gly Gly Ser Gly Gly Gly Asp Val Pro Leu 
65                  70                  75                  80  

Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn Phe Asp Lys Lys 
                85                  90                  95      

Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu Leu Trp Gly Pro 
            100                 105                 110         

Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly Lys Ile Leu Arg 
        115                 120                 125             

Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe Gln Val Pro Glu 
    130                 135                 140                 

Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu Gly Phe Ala Phe 
145                 150                 155                 160 

His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile Ser Gly Thr Phe 
                165                 170                 175     

Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn Gln Thr Ile Ile 
            180                 185                 190         

Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu Glu Lys Pro Val 
        195                 200                 205             

Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His Gln Ser Gly Arg 
    210                 215                 220                 

Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr Ile Gly Asp Gln 
225                 230                 235                 240 

Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn Gln Ala Gln His 
                245                 250                 255     

Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr His Thr Tyr Met 
            260                 265                 270         

Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile Pro Lys Asp Asn 
        275                 280                 285             

Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr Leu Gly His Arg 
    290                 295                 300                 

Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys Leu Leu Gln Ser 
305                 310                 315                 320 

Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu Ile Val Lys Gly 
                325                 330                 335     

Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys Asp Asp Ser Gly 
            340                 345                 350         

Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys Thr Ile Lys Asp 
        355                 360                 365             

Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val Pro Val Thr Lys 
    370                 375                 380                 

Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro Leu Lys Thr Leu 
385                 390                 395                 400 

Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro Thr Cys Gly Glu 
                405                 410                 415     

Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser Ser Ala Tyr Val 
            420                 425                 430         

Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu Asn Thr Leu Leu 
        435                 440                 445             

Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile Lys Leu Asp Pro 
    450                 455                 460                 

Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met Phe Lys Ser Gly 
465                 470                 475                 480 

Cys Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser 
                485                 490                 495     

Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly 
            500                 505                 510         

Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln 
        515                 520                 525             

Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe 
    530                 535                 540                 

Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys Asp Thr Asp Ser 
545                 550                 555                 560 

Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp Lys Asp Gly Asn 
               565                 570                 575     

Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly 
            580                 585                 590         

Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp 
        595                 600                 605             

Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe Val Gln Met Met 
    610                 615                 620                 

Thr Ala Gly Gly Ser Cys Gly Asn Arg Tyr Arg Asp Val Ile Ala Ser 
625                 630                 635                 640 

Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr Ala Gly Asn Val 
                645                 650                 655     

Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu Asn Pro Gly Ser 
            660                 665                 670         

Leu Ile Lys Phe Thr Tyr Lys Ala Lys Lys Leu Ala Ala Ala Leu Glu 
        675                 680                 685             

His His His His His His 
    690                 


<210>  64
<211>  169
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5th generation human serum albumin (HAS) sensor component 2 (VHH 
       binder-Cam-BP)

<400>  64

Asp Gly His His His His His His Gly Ser Gly Gln Val Gln Leu Gln 
1               5                   10                  15      

Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly Ser Leu Arg Leu Ser 
            20                  25                  30          

Cys Ala Ala Ser Gly Tyr Ile Ser Asp Ala Tyr Tyr Met Gly Trp Tyr 
        35                  40                  45              

Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Ala Thr Ile Thr His 
    50                  55                  60                  

Gly Thr Asn Thr Tyr Tyr Ala Asp Ser Val Lys Gly Arg Phe Thr Ile 
65                  70                  75                  80  

Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu Gln Met Asn Ser Leu 
                85                  90                  95      

Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Val Leu Glu Thr Arg 
            100                 105                 110         

Ser Tyr Ser Phe Arg Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             

Ser Gly Gly Ser Gly Ser Gly Gly Ser Gly Gly Gly Ser Gly Ser Ser 
    130                 135                 140                 

Gly Gly Ser Gly Gly Ser Gly Gly Gly Lys Arg Arg Trp Lys Lys Asn 
145                 150                 155                 160 

Phe Ile Ala Val Ser Ala Ala Asn Arg 
                165                 

<210>  65
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GA binder (comprised within GA binder-GDH-CalM)

<400>  65

Ala Val Asp Ala Asn Ser Leu Ala Glu Ala Lys Val Leu Ala Asn Arg 
1               5                   10                  15      

Glu Leu Asp Lys Tyr Gly Val Ser Asp Phe Tyr Lys Arg Leu Ile Asn 
            20                  25                  30          

Lys Ala Lys Thr Val Glu Gly Val Glu Ala Leu Lys Leu His Ile Leu 
        35                  40                  45              

Ala Ala Leu Pro 
    50          


<210>  66
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH binder (comprised within VHH binder-Cam-BP)

<400>  66

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      

Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Tyr Ile Ser Asp Ala Tyr 
            20                  25                  30          

Tyr Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              

Ala Thr Ile Thr His Gly Thr Asn Thr Tyr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  

Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  

Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      

Val Leu Glu Thr Arg Ser Tyr Ser Phe Arg Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         

Gln Val Thr Val Ser Ser 
        115             


<210>  67
<211>  798
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  5th generation cyclosporine sensor component 1 
       (GDH-CalM-Cyclophilin)

<400>  67

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 


Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 

Phe Lys Ser Gly Cys Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys 
                405                 410                 415     

Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
            420                 425                 430         

Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
        435                 440                 445             

Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
    450                 455                 460                 

Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
465                 470                 475                 480 

Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
                485                 490                 495     

Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met 
            500                 505                 510         

Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
        515                 520                 525             

Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
    530                 535                 540                 

Val Gln Met Met Thr Ala Gly Gly Ser Cys Gly Asn Arg Tyr Arg Asp 
545                 550                 555                 560 

Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr 
                565                 570                 575     

Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu 
            580                 585                 590         

Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys Gly Gly Ser 
        595                 600                 605             

Gly Gly Ser Gly Ser Gly Ser Gly Gly Ser Gly Gly Met Val Asn Pro 
    610                 615                 620                 

Thr Val Phe Phe Asp Ile Ala Val Asp Gly Glu Pro Leu Gly Arg Val 
625                 630                 635                 640 

Ser Phe Glu Leu Phe Ala Asp Lys Val Pro Lys Thr Ala Glu Asn Phe 
                645                 650                 655     

Arg Ala Leu Ser Thr Gly Glu Lys Gly Phe Gly Tyr Lys Gly Ser Cys 
            660                 665                 670         

Phe His Arg Ile Ile Pro Gly Phe Met Cys Gln Gly Gly Asp Phe Thr 
        675                 680                 685             

Arg His Asn Gly Thr Gly Gly Lys Ser Ile Tyr Gly Glu Lys Phe Glu 
    690                 695                 700                 

Asp Glu Asn Phe Ile Leu Lys His Thr Gly Pro Gly Ile Leu Ser Met 
705                 710                 715                 720 

Ala Asn Ala Gly Pro Asn Thr Asn Gly Ser Gln Phe Phe Ile Cys Thr 
                725                 730                 735     

Ala Lys Thr Glu Trp Leu Asp Gly Lys His Val Val Phe Gly Lys Val 
            740                 745                 750         

Lys Glu Gly Met Asn Ile Val Glu Ala Met Glu Arg Phe Gly Ser Arg 
        755                 760                 765             

Asn Gly Lys Thr Ser Lys Lys Ile Thr Ile Ala Asp Cys Gly Gln Leu 
    770                 775                 780                 

Glu Lys Leu Ala Ala Ala Leu Glu His His His His His His 
785                 790                 795             


<210>  68
<211>  165
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cyclophilin (comprised within GDH-CalM-Cyclophilin)

<400>  68

Met Val Asn Pro Thr Val Phe Phe Asp Ile Ala Val Asp Gly Glu Pro 
1               5                   10                  15      

Leu Gly Arg Val Ser Phe Glu Leu Phe Ala Asp Lys Val Pro Lys Thr 
            20                  25                  30          

Ala Glu Asn Phe Arg Ala Leu Ser Thr Gly Glu Lys Gly Phe Gly Tyr 
        35                  40                  45              

Lys Gly Ser Cys Phe His Arg Ile Ile Pro Gly Phe Met Cys Gln Gly 
    50                  55                  60                  

Gly Asp Phe Thr Arg His Asn Gly Thr Gly Gly Lys Ser Ile Tyr Gly 
65                  70                  75                  80  

Glu Lys Phe Glu Asp Glu Asn Phe Ile Leu Lys His Thr Gly Pro Gly 
                85                  90                  95      

Ile Leu Ser Met Ala Asn Ala Gly Pro Asn Thr Asn Gly Ser Gln Phe 
            100                 105                 110         

Phe Ile Cys Thr Ala Lys Thr Glu Trp Leu Asp Gly Lys His Val Val 
        115                 120                 125             

Phe Gly Lys Val Lys Glu Gly Met Asn Ile Val Glu Ala Met Glu Arg 
    130                 135                 140                 

Phe Gly Ser Arg Asn Gly Lys Thr Ser Lys Lys Ile Thr Ile Ala Asp 
145                 150                 155                 160 

Cys Gly Gln Leu Glu 
                165 

<210>  69
<211>  664
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GDH-affinity clamp chimer (GDH-Affinity Clamp)

<400>  69

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 

Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Glu Asp Ala Pro Glu Ser Gly 
                325                 330                 335     

Ser Pro Glu Leu Gly Phe Ser Ile Ser Gly Gly Val Gly Gly Arg Gly 
            340                 345                 350         

Asn Pro Phe Arg Pro Asp Asp Asp Gly Ile Phe Val Thr Arg Val Gln 
        355                 360                 365             

Pro Glu Gly Pro Ala Ser Lys Leu Leu Gln Pro Gly Asp Lys Ile Ile 
    370                 375                 380                 

Gln Ala Asn Gly Tyr Ser Phe Ile Asn Ile Glu His Gly Gln Ala Val 
385                 390                 395                 400 

Ser Leu Leu Lys Thr Phe Gln Asn Thr Val Glu Leu Ile Ile Val Arg 
                405                 410                 415     

Glu Val Gly Asn Gly Ala Lys Gln Glu Ile Arg Val Arg Val Glu Lys 
            420                 425                 430         

Asp Gly Gly Ser Gly Gly Val Ser Ser Val Pro Thr Asn Leu Glu Val 
        435                 440                 445             

Val Ala Ala Thr Pro Thr Ser Leu Leu Ile Ser Trp Asp Ala Tyr Arg 
    450                 455                 460                 

Glu Leu Pro Val Ser Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly 
465                 470                 475                 480 

Asn Ser Pro Val Gln Glu Phe Thr Val Pro Gly Ser Lys Ser Thr Ala 
                485                 490                 495     

Thr Ile Ser Gly Leu Lys Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr 
            500                 505                 510         

Ala His Tyr Asn Tyr His Tyr Tyr Ser Ser Pro Ile Ser Ile Asn Tyr 
        515                 520                 525             

Arg Gly Ser Gly Pro Gly Tyr Asn Tyr Asn Asp Pro Thr Cys Gly Glu 
    530                 535                 540                 

Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser Ser Ala Tyr Val 
545                 550                 555                 560 

Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu Asn Thr Leu Leu 
                565                 570                 575     

Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile Lys Leu Asp Pro 
            580                 585                 590         

Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met Phe Lys Ser Asn 
        595                 600                 605             

Asn Arg Tyr Arg Asp Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr 
    610                 615                 620                 

Val Leu Thr Asp Thr Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val 
625                 630                 635                 640 

Thr Asn Thr Leu Glu Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys 
                645                 650                 655     

Ala Lys His His His His His His 
            660                 

<210>  70
<211>  322
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  1st generation CalM-DHFR chimer

<400>  70

Ile Ser Leu Ile Ala Ala Leu Ala Val Asp Arg Val Ile Gly Met Glu 
1               5                   10                  15      

Asn Ala Met Pro Trp Asn Leu Pro Ala Asp Leu Ala Trp Phe Lys Arg 
            20                  25                  30          

Asn Thr Leu Asn Lys Pro Val Ile Met Gly Arg His Thr Trp Glu Ser 
        35                  40                  45              

Ile Gly Arg Pro Leu Pro Gly Arg Lys Asn Ile Ile Leu Ser Ser Gln 
    50                  55                  60                  

Pro Gly Thr Asp Asp Arg Val Thr Trp Val Lys Ser Val Asp Glu Ala 
65                  70                  75                  80  

Ile Ala Ala Cys Gly Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala Glu 
                85                  90                  95      

Phe Lys Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile 
            100                 105                 110         

Thr Thr Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro 
        115                 120                 125             

Thr Glu Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly 
    130                 135                 140                 

Asn Gly Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys 
145                 150                 155                 160 

Met Lys Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val 
                165                 170                 175     

Phe Asp Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His 
      	   180                  185                 190  	

Val Met Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu 
        195                 200                 205             

Met Ile Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu 
    210                 215                 220                 

Glu Phe Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Val Pro Glu 
225                 230                 235                 240 

Ile Met Val Ile Gly Gly Gly Arg Val Tyr Glu Gln Phe Leu Pro Lys 
                245                 250                 255     

Ala Gln Lys Leu Tyr Leu Thr His Ile Asp Ala Glu Val Glu Gly Asp 
            260                 265                 270         

Thr His Phe Pro Asp Tyr Glu Pro Asp Asp Trp Glu Ser Val Phe Ser 
        275                 280                 285             

Glu Phe His Asp Ala Asp Ala Gln Asn Ser His Ser Tyr Cys Phe Glu 
    290                 295                 300                 

Ile Leu Glu Arg Arg Lys Leu Ala Ala Ala Leu Glu His His His His 
305                 310                 315                 320 

His His 
        

<210>  71
<211>  164
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DHFR (comprised within the CalM-DHFR)

<400>  71

Ile Ser Leu Ile Ala Ala Leu Ala Val Asp Arg Val Ile Gly Met Glu 
1               5                   10                  15      

Asn Ala Met Pro Trp Asn Leu Pro Ala Asp Leu Ala Trp Phe Lys Arg 
            20                  25                  30          

Asn Thr Leu Asn Lys Pro Val Ile Met Gly Arg His Thr Trp Glu Ser 
        35                  40                  45              

Ile Gly Arg Pro Leu Pro Gly Arg Lys Asn Ile Ile Leu Ser Ser Gln 
    50                  55                  60                  

Pro Gly Thr Asp Asp Arg Val Thr Trp Val Lys Ser Val Asp Glu Ala 
65                  70                  75                  80  

Ile Ala Ala Cys Gly Val Pro Glu Ile Met Val Ile Gly Gly Gly Arg 
                85                  90                  95      

Val Tyr Glu Gln Phe Leu Pro Lys Ala Gln Lys Leu Tyr Leu Thr His 
            100                 105                 110         

Ile Asp Ala Glu Val Glu Gly Asp Thr His Phe Pro Asp Tyr Glu Pro 
        115                 120                 125             

Asp Asp Trp Glu Ser Val Phe Ser Glu Phe His Asp Ala Asp Ala Gln 
    130                 135                 140                 

Asn Ser His Ser Tyr Cys Phe Glu Ile Leu Glu Arg Arg Lys Leu Ala 
145                 150                 155                 160 

Ala Ala Leu Glu 
                

<210>  72
<211>  795
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Three component system - amylase sensor - component 1: 5th 
       generation GDH-CaM-SH3-FKBP

<400>  72

Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn 
1               5                   10                  15      

Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu 
            20                  25                  30          

Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly 
        35                  40                  45              

Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe 
    50                  55                  60                  

Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu 
65                  70                  75                  80  

Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile 
                85                  90                  95      

Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn 
            100                 105                 110         

Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu 
        115                 120                 125             

Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His 
    130                 135                 140                 

Gln Ser Gly Arg Leu Val Ile Gly Pro Asp Gln Lys Ile Tyr Tyr Thr 
145                 150                 155                 160 

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
                165                 170                 175     

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
            180                 185                 190         

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
        195                 200                 205             

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
    210                 215                 220                 


Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
225                 230                 235                 240 

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
                245                 250                 255     

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
            260                 265                 270         

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
        275                 280                 285             

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
    290                 295                 300                 

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
305                 310                 315                 320 

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
                325                 330                 335     

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
            340                 345                 350         

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
        355                 360                 365             

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
    370                 375                 380                 

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
385                 390                 395                 400 

Phe Lys Ser Gly Cys Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys 
                405                 410                 415     

Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
            420                 425                 430         

Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
        435                 440                 445             

Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
    450                 455                 460                 

Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
465                 470                 475                 480 

Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
                485                 490                 495     

Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met 
            500                 505                 510         

Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
        515                 520                 525             

Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
    530                 535                 540                 

Val Gln Met Met Thr Ala Gly Gly Ser Cys Gly Asn Arg Tyr Arg Asp 
545                 550                 555                 560 

Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr 
                565                 570                 575     

Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu 
            580                 585                 590         

Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys Gly Ser Gly 
        595                 600                 605             

Ser Gly Gly Ala Glu Tyr Val Arg Ala Leu Phe Asp Phe Asn Gly Asn 
    610                 615                 620                 

Asp Glu Glu Asp Leu Pro Phe Lys Lys Gly Asp Ile Leu Arg Ile Arg 
625                 630                 635                 640 

Asp Lys Pro Glu Glu Gln Trp Trp Asn Ala Glu Asp Ser Glu Gly Lys 
                645                 650                 655     

Arg Gly Met Ile Pro Val Pro Tyr Val Glu Lys Tyr Gly Gly Ser Gly 
            660                 665                 670         

Gly Ser Gly Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
        675                 680                 685             

Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
    690                 695                 700                 

Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro 
705                 710                 715                 720 

Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
                725                 730                 735     

Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
            740                 745                 750         

Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
        755                 760                 765             

His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Lys Leu 
    770                 775                 780                 

Ala Ala Ala Leu Glu His His His His His His 
785                 790                 795 

<210>  73
<211>  57
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SH3

<400>  73

Ala Glu Tyr Val Arg Ala Leu Phe Asp Phe Asn Gly Asn Asp Glu Glu 
1               5                   10                  15      

Asp Leu Pro Phe Lys Lys Gly Asp Ile Leu Arg Ile Arg Asp Lys Pro 
            20                  25                  30          

Glu Glu Gln Trp Trp Asn Ala Glu Asp Ser Glu Gly Lys Arg Gly Met 
        35                  40                  45              

Ile Pro Val Pro Tyr Val Glu Lys Tyr 
    50                  55          


<210>  74
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FKBP

<400>  74

Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys 
1               5                   10                  15      

Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly 
            20                  25                  30          

Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met 
        35                  40                  45              

Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln 
    50                  55                  60                  

Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala 
65                  70                  75                  80  

Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr Leu 
                85                  90                  95      

Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105     

<210>  75
<211>  203
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Three component system - amylase sensor - component 2: 
       FRB-SH3L-VHH1

<400>  75

Ser His His His His His His Gly Thr Arg Val Ala Ile Leu Trp His 
1               5                   10                  15      

Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly 
            20                  25                  30          

Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro Leu His Ala 
        35                  40                  45              

Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln 
    50                  55                  60                  

Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr 
65                  70                  75                  80  

Met Lys Ser Gly Asn Val Lys Asp Leu Thr Gln Ala Trp Asp Leu Tyr 
                85                  90                  95      

Tyr His Val Phe Arg Arg Ile Ser Gly Gly Ser Gly Gly Ser Gly Gly 
            100                 105                 110         

Pro Pro Pro Pro Leu Pro Pro Lys Arg Arg Arg Gly Gly Ser Gly Ser 
        115                 120                 125             

Gly Asp Thr Thr Val Ser Glu Pro Ala Pro Ser Cys Val Thr Leu Tyr 
    130                 135                 140                 

Gln Ser Trp Arg Tyr Ser Gln Ala Asp Asn Gly Cys Ala Glu Thr Val 
145                 150                 155                 160 

Thr Val Lys Val Val Tyr Glu Asp Asp Thr Glu Gly Leu Cys Tyr Ala 
                165                 170                 175     

Val Ala Pro Gly Gln Ile Thr Thr Val Gly Asp Gly Tyr Ile Gly Ser 
            180                 185                 190         

His Gly His Ala Arg Tyr Leu Ala Arg Cys Leu 
        195                 200             

<210>  76
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SH3L

<400>  76

Pro Pro Pro Pro Leu Pro Pro Lys Arg Arg Arg 
1               5                   10      


<210>  77
<211>  163
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Three component system - amylase sensor - component 3: VHH2-CaMBP

<400>  77

Asp Gly His His His His His His Gly Ser Gly Gln Val Gln Leu Val 
1               5                   10                  15      

Glu Ser Gly Gly Gly Thr Val Pro Ala Gly Gly Ser Leu Arg Leu Ser 
            20                  25                  30          

Cys Ala Ala Ser Gly Asn Thr Leu Cys Thr Tyr Asp Met Ser Trp Tyr 
        35                  40                  45              

Arg Arg Ala Pro Gly Lys Gly Arg Asp Phe Val Ser Gly Ile Asp Asn 
    50                  55                  60                  

Asp Gly Thr Thr Thr Tyr Val Asp Ser Val Ala Gly Arg Phe Thr Ile 
65                  70                  75                  80  

Ser Gln Gly Asn Ala Lys Asn Thr Ala Tyr Leu Gln Met Asp Ser Leu 
                85                  90                  95      

Lys Pro Asp Asp Thr Ala Met Tyr Tyr Cys Lys Pro Ser Leu Arg Tyr 
            100                 105                 110         

Gly Leu Pro Gly Cys Pro Ile Ile Pro Trp Gly Gln Gly Thr Gln Val 
        115                 120                 125             

Thr Val Ser Ser Gly Gly Ser Gly Gly Ser Gly Ser Gly Ser Gly Gly 
    130                 135                 140                 

Ser Gly Gly Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala 
145                 150                 155                 160 

Ala Asn Arg 
          

<210>  78
<211>  843
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single component system - rapamycin sensor - FKBP-GDH-CaM-FRB

<400>  78

Asp His His His His His His Gly Val Gln Val Glu Thr Ile Ser Pro 
1               5                   10                  15      

Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His 
            20                  25                  30          

Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Phe Asp Ser Ser Arg Asp 
        35                  40                  45              

Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg 
    50                  55                  60                  

Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys 
65                  70                  75                  80  

Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly 
                85                  90                  95      

Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys 
            100                 105                 110         

Leu Glu Gly Gly Ser Gly Gly Ser Gly Gly Gln Lys Ile Tyr Tyr Thr 
        115                 120                 125             

Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro Asn 
    130                 135                 140                 

Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp Tyr 
145                 150                 155                 160 

His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser Ile 
                165                 170                 175     

Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr Thr 
            180                 185                 190         

Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly Lys 
        195                 200                 205             

Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn Leu 
    210                 215                 220                 

Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr Lys 
225                 230                 235                 240 

Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn Lys 
                245                 250                 255     

Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly Val 
            260                 265                 270         

Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro Pro 
        275                 280                 285             

Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp Pro 
    290                 295                 300                 

Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro Ser 
305                 310                 315                 320 

Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp Glu 
                325                 330                 335     

Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg Ile 
            340                 345                 350         

Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro Met 
        355                 360                 365             

Phe Lys Ser Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe Lys 
    370                 375                 380                 

Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr 
385                 390                 395                 400 

Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu 
                405                 410                 415     

Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly 
            420                 425                 430         

Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys 
        435                 440                 445             

Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp 
    450                 455                 460                 

Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met 
465                 470                 475                 480 

Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile 
                485                 490                 495     

Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe 
            500                 505                 510         

Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Asn Arg Tyr Arg Asp 
        515                 520                 525             

Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr 
    530                 535                 540                 

Ala Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu 
545                 550                 555                 560 

Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys Gly Gly Ser 
                565                 570                 575     

Gly Ser Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly Ser Gly Gly Asp 
            580                 585                 590         

Val Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn Phe 
        595                 600                 605             

Asp Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu Leu 
    610                 615                 620                 

Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly Lys 
625                 630                 635                 640 

Ile Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe Gln 
                645                 650                 655     

Val Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu Gly 
            660                 665                 670         

Phe Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile Ser 
        675                 680                 685             

Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn Gln 
    690                 695                 700                 

Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu Glu 
705                 710                 715                 720 

Lys Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His Gln 
                725                 730                 735     

Ser Gly Arg Leu Val Ile Gly Pro Gly Ser Gly Ser Gly Ser Gly Gly 
            740                 745                 750         

Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu 
        755                 760                 765             

Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro 
    770                 775                 780                 

Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser 
785                 790                 795                 800 

Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys 
                805                 810                 815     

Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Thr Gln Ala Trp 
            820                 825                 830         

Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser 
        835                 840             


<210>  79
<211>  471
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Circularly permutated GDH for single component system

<400>  79

Gln Lys Ile Tyr Tyr Thr Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala 
1               5                   10                  15      

Tyr Leu Phe Leu Pro Asn Gln Ala Gln His Thr Pro Thr Gln Gln Glu 
            20                  25                  30          

Leu Asn Gly Lys Asp Tyr His Thr Tyr Met Gly Lys Val Leu Arg Leu 
        35                  40                  45              

Asn Leu Asp Gly Ser Ile Pro Lys Asp Asn Pro Ser Phe Asn Gly Val 
    50                  55                  60                  

Val Ser His Ile Tyr Thr Leu Gly His Arg Asn Pro Gln Gly Leu Ala 
65                  70                  75                  80  

Phe Thr Pro Asn Gly Lys Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser 
                85                  90                  95      

Asp Asp Glu Ile Asn Leu Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro 
            100                 105                 110         

Asn Val Ala Gly Tyr Lys Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr 
        115                 120                 125             

Ser Ala Ala Ala Asn Lys Thr Ile Lys Asp Leu Ala Gln Asn Gly Val 
    130                 135                 140                 

Lys Val Ala Ala Gly Val Pro Val Thr Lys Glu Ser Glu Trp Thr Gly 
145                 150                 155                 160 

Lys Asn Phe Val Pro Pro Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr 
                165                 170                 175     

Tyr Asn Tyr Asn Asp Pro Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp 
            180                 185                 190         

Pro Thr Val Ala Pro Ser Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys 
        195                 200                 205             

Ala Ile Thr Gly Trp Glu Asn Thr Leu Leu Val Pro Ser Leu Lys Arg 
    210                 215                 220                 

Gly Val Ile Phe Arg Ile Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr 
225                 230                 235                 240 

Asp Asp Ala Val Pro Met Phe Lys Ser Asn Asn Arg Tyr Arg Asp Val 
                245                 250                 255     

Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr Asp Thr Ala 
            260                 265                 270         

Gly Asn Val Gln Lys Asp Asp Gly Ser Val Thr Asn Thr Leu Glu Asn 
        275                 280                 285             

Pro Gly Ser Leu Ile Lys Phe Thr Tyr Lys Ala Lys Gly Gly Ser Gly 
    290                 295                 300                 

Ser Gly Ser Gly Ser Gly Gly Ser Gly Ser Gly Ser Gly Gly Asp Val 
305                 310                 315                 320 

Pro Leu Ile Pro Ser Gln Phe Ala Lys Ala Lys Ser Glu Asn Phe Asp 
                325                 330                 335     

Lys Lys Val Ile Leu Ser Asn Leu Asn Lys Pro His Ala Leu Leu Trp 
            340                 345                 350         

Gly Pro Asp Asn Gln Ile Trp Leu Thr Glu Arg Ala Thr Gly Lys Ile 
        355                 360                 365             

Leu Arg Val Asn Pro Glu Ser Gly Ser Val Lys Thr Val Phe Gln Val 
    370                 375                 380                 

Pro Glu Ile Val Asn Asp Ala Asp Gly Gln Asn Gly Leu Leu Gly Phe 
385                 390                 395                 400 

Ala Phe His Pro Asp Phe Lys Asn Asn Pro Tyr Ile Tyr Ile Ser Gly 
                405                 410                 415     

Thr Phe Lys Asn Pro Lys Ser Thr Asp Lys Glu Leu Pro Asn Gln Thr 
            420                 425                 430         

Ile Ile Arg Arg Tyr Thr Tyr Asn Lys Ser Thr Asp Thr Leu Glu Lys 
        435                 440                 445             

Pro Val Asp Leu Leu Ala Gly Leu Pro Ser Ser Lys Asp His Gln Ser 
    450                 455                 460                 

Gly Arg Leu Val Ile Gly Pro 
465                 470     


<210>  80
<211>  91
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FRB in single component rapamycin sensor

<400>  80

Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg Leu 
1               5                   10                  15      

Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu Pro 
            20                  25                  30          

Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr Ser 
        35                  40                  45              

Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp Cys 
    50                  55                  60                  

Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Thr Gln Ala Trp 
65                  70                  75                  80  

Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser 
                85                  90      

<210>  81
<211>  1323
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single component system - FK506 sensor - calcineurin 
       alpha/beta-GDH-CaM-FKBP

<400>  81

Asp Ser Ser Gly Thr Ser Glu Pro Lys Ala Ile Asp Pro Lys Leu Ser 
1               5                   10                  15      

Thr Thr Asp Arg Val Val Lys Ala Val Pro Phe Pro Pro Ser His Arg 
            20                  25                  30          

Leu Thr Ala Lys Glu Val Phe Asp Asn Asp Gly Lys Pro Arg Val Asp 
        35                  40                  45              

Ile Leu Lys Ala His Leu Met Lys Glu Gly Arg Leu Glu Glu Ser Val 
    50                  55                  60                  

Ala Leu Arg Ile Ile Thr Glu Gly Ala Ser Ile Leu Arg Gln Glu Lys 
65                  70                  75                  80  

Asn Leu Leu Asp Ile Asp Ala Pro Val Thr Val Cys Gly Asp Ile His 
                85                  90                  95      

Gly Gln Phe Phe Asp Leu Met Lys Leu Phe Glu Val Gly Gly Ser Pro 
            100                 105                 110         

Ala Asn Thr Arg Tyr Leu Phe Leu Gly Asp Tyr Val Asp Arg Gly Tyr 
        115                 120                 125             

Phe Ser Ile Glu Cys Val Leu Tyr Leu Trp Ala Leu Lys Ile Leu Tyr 
    130                 135                 140                 

Pro Lys Thr Leu Phe Leu Leu Arg Gly Asn His Glu Cys Arg His Leu 
145                 150                 155                 160 

Thr Glu Tyr Phe Thr Phe Lys Gln Glu Cys Lys Ile Lys Tyr Ser Glu 
                165                 170                 175     

Arg Val Tyr Asp Ala Cys Met Asp Ala Phe Asp Cys Leu Pro Leu Ala 
            180                 185                 190         

Ala Leu Met Asn Gln Gln Phe Leu Cys Val His Gly Gly Leu Ser Pro 
        195                 200                 205             

Glu Ile Asn Thr Leu Asp Asp Ile Arg Lys Leu Asp Arg Phe Lys Glu 
    210                 215                 220                 

Pro Pro Ala Tyr Gly Pro Met Cys Asp Ile Leu Trp Ser Asp Pro Leu 
225                 230                 235                 240 

Glu Asp Phe Gly Asn Glu Lys Thr Gln Glu His Phe Thr His Asn Thr 
                245                 250                 255     

Val Arg Gly Cys Ser Tyr Phe Tyr Ser Tyr Pro Ala Val Cys Glu Phe 
            260                 265                 270         

Leu Gln His Asn Asn Leu Leu Ser Ile Leu Arg Ala His Glu Ala Gln 
        275                 280                 285             

Asp Ala Gly Tyr Arg Met Tyr Arg Lys Ser Gln Thr Thr Gly Phe Pro 
    290                 295                 300                 

Ser Leu Ile Thr Ile Phe Ser Ala Pro Asn Tyr Leu Asp Val Tyr Asn 
305                 310                 315                 320 

Asn Lys Ala Ala Val Leu Lys Tyr Glu Asn Asn Val Met Asn Ile Arg 
                325                 330                 335     

Gln Phe Asn Cys Ser Pro His Pro Tyr Trp Leu Pro Asn Phe Met Asp 
            340                 345                 350         

Val Phe Thr Trp Ser Leu Pro Phe Val Gly Glu Lys Val Thr Glu Met 
        355                 360                 365             

Leu Val Asn Val Leu Asn Ile Cys Ser Asp Asp Glu Leu Gly Ser Glu 
    370                 375                 380                 

Glu Asp Gly Ser Gly Ser Gly Ser Gly Gly Gly Asn Glu Ala Ser Tyr 
385                 390                 395                 400 

Pro Leu Glu Met Cys Ser His Phe Asp Ala Asp Glu Ile Lys Arg Leu 
                405                 410                 415     

Gly Lys Arg Phe Lys Lys Leu Asp Leu Asp Asn Ser Gly Ser Leu Ser 
            420                 425                 430         

Val Glu Glu Phe Met Ser Leu Pro Glu Leu Gln Gln Asn Pro Leu Val 
        435                 440                 445             

Gln Arg Val Ile Asp Ile Phe Asp Thr Asp Gly Asn Gly Glu Val Asp 
    450                 455                 460                 

Phe Lys Glu Phe Ile Glu Gly Val Ser Gln Phe Ser Val Lys Gly Asp 
465                 470                 475                 480 

Lys Glu Gln Lys Leu Arg Phe Ala Phe Arg Ile Tyr Asp Met Asp Lys 
                485                 490                 495     

Asp Gly Tyr Ile Ser Asn Gly Glu Leu Phe Gln Val Leu Lys Met Met 
            500                 505                 510         

Val Gly Asn Asn Leu Lys Asp Thr Gln Leu Gln Gln Ile Val Asp Lys 
        515                 520                 525             

Thr Ile Ile Asn Ala Asp Lys Asp Gly Asp Gly Arg Ile Ser Phe Glu 
    530                 535                 540                 

Glu Phe Cys Ala Val Val Gly Gly Leu Asp Ile His Lys Lys Met Val 
545                 550                 555                 560 

Val Asp Val Gly Gly Ser Gly Ser Gly Ser Gly Gly Gln Lys Ile Tyr 
                565                 570                 575     

Tyr Thr Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu 
            580                 585                 590         

Pro Asn Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys 
        595                 600                 605             

Asp Tyr His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly 
    610                 615                 620                 

Ser Ile Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile 
625                 630                 635                 640 

Tyr Thr Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn 
                645                 650                 655     

Gly Lys Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile 
            660                 665                 670         

Asn Leu Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly 
        675                 680                 685             

Tyr Lys Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala 
    690                 695                 700                 

Asn Lys Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala 
705                 710                 715                 720 

Gly Val Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val 
                725                 730                 735     

Pro Pro Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn 
            740                 745                 750         

Asp Pro Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala 
        755                 760                 765             

Pro Ser Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly 
    770                 775                 780                 

Trp Glu Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe 
785                 790                 795                 800 

Arg Ile Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val 
                805                 810                 815     

Pro Met Phe Lys Ser Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala Glu 
            820                 825                 830         

Phe Lys Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile 
        835                 840                 845             

Thr Thr Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro 
    850                 855                 860                 

Thr Glu Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly 
865                 870                 875                 880 

Asn Gly Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys 
                885                 890                 895     

Met Lys Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val 
            900                 905                 910         

Phe Asp Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His 
        915                 920                 925             

Val Met Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu 
    930                 935                 940                 

Met Ile Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu 
945                 950                 955                 960 

Glu Phe Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Asn Arg Tyr 
                965                 970                 975     

Arg Asp Val Ile Ala Ser Pro Asp Gly Asn Val Leu Tyr Val Leu Thr 
            980                 985                 990         

Asp Thr Ala Gly Asn Val Gln Lys  Asp Asp Gly Ser Val  Thr Asn Thr 
        995                 1000                 1005             

Leu Glu  Asn Pro Gly Ser Leu  Ile Lys Phe Thr Tyr  Lys Ala Lys 
    1010                 1015                 1020             

Gly Gly  Ser Gly Ser Gly Ser  Gly Ser Gly Gly Ser  Gly Ser Gly 
    1025                 1030                 1035             

Ser Gly  Gly Asp Val Pro Leu  Ile Pro Ser Gln Phe  Ala Lys Ala 
    1040                 1045                 1050             

Lys Ser  Glu Asn Phe Asp Lys  Lys Val Ile Leu Ser  Asn Leu Asn 
    1055                 1060                 1065             

Lys Pro  His Ala Leu Leu Trp  Gly Pro Asp Asn Gln  Ile Trp Leu 
    1070                 1075                 1080             

Thr Glu  Arg Ala Thr Gly Lys  Ile Leu Arg Val Asn  Pro Glu Ser 
    1085                 1090                 1095             

Gly Ser  Val Lys Thr Val Phe  Gln Val Pro Glu Ile  Val Asn Asp 
    1100                 1105                 1110             

Ala Asp  Gly Gln Asn Gly Leu  Leu Gly Phe Ala Phe  His Pro Asp 
    1115                 1120                 1125             

Phe Lys  Asn Asn Pro Tyr Ile  Tyr Ile Ser Gly Thr  Phe Lys Asn 
    1130                 1135                 1140             

Pro Lys  Ser Thr Asp Lys Glu  Leu Pro Asn Gln Thr  Ile Ile Arg 
    1145                 1150                 1155             

Arg Tyr  Thr Tyr Asn Lys Ser  Thr Asp Thr Leu Glu  Lys Pro Val 
    1160                 1165                 1170             

Asp Leu  Leu Ala Gly Leu Pro  Ser Ser Lys Asp His  Gln Ser Gly 
    1175                 1180                 1185             

Arg Leu  Val Ile Gly Pro Gly  Ser Gly Ser Ser Gly  Ser Gly Gly 
    1190                 1195                 1200             

Gly Val  Gln Val Glu Thr Ile  Ser Pro Gly Asp Gly  Arg Thr Phe 
    1205                 1210                 1215             

Pro Lys  Arg Gly Gln Thr Cys  Val Val His Tyr Thr  Gly Met Leu 
    1220                 1225                 1230             

Glu Asp  Gly Lys Lys Phe Asp  Ser Ser Arg Asp Arg  Asn Lys Pro 
    1235                 1240                 1245             

Phe Lys  Phe Met Leu Gly Lys  Gln Glu Val Ile Arg  Gly Trp Glu 
    1250                 1255                 1260             

Glu Gly  Val Ala Gln Met Ser  Val Gly Gln Arg Ala  Lys Leu Thr 
    1265                 1270                 1275             

Ile Ser  Pro Asp Tyr Ala Tyr  Gly Ala Thr Gly His  Pro Gly Ile 
    1280                 1285                 1290             

Ile Pro  Pro His Ala Thr Leu  Val Phe Asp Val Glu  Leu Leu Lys 
    1295                 1300                 1305             

Leu Glu  Lys Leu Ala Ala Ala  Leu Glu His His His  His His His 
    1310                 1315                 1320             


<210>  82
<211>  385
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single component system - FK506 sensor - calcineurin alpha

<400>  82

Ser Ser Gly Thr Ser Glu Pro Lys Ala Ile Asp Pro Lys Leu Ser Thr 
1               5                   10                  15      

Thr Asp Arg Val Val Lys Ala Val Pro Phe Pro Pro Ser His Arg Leu 
            20                  25                  30          

Thr Ala Lys Glu Val Phe Asp Asn Asp Gly Lys Pro Arg Val Asp Ile 
        35                  40                  45              

Leu Lys Ala His Leu Met Lys Glu Gly Arg Leu Glu Glu Ser Val Ala 
    50                  55                  60                  

Leu Arg Ile Ile Thr Glu Gly Ala Ser Ile Leu Arg Gln Glu Lys Asn 
65                  70                  75                  80  

Leu Leu Asp Ile Asp Ala Pro Val Thr Val Cys Gly Asp Ile His Gly 
                85                  90                  95      

Gln Phe Phe Asp Leu Met Lys Leu Phe Glu Val Gly Gly Ser Pro Ala 
            100                 105                 110         

Asn Thr Arg Tyr Leu Phe Leu Gly Asp Tyr Val Asp Arg Gly Tyr Phe 
        115                 120                 125             

Ser Ile Glu Cys Val Leu Tyr Leu Trp Ala Leu Lys Ile Leu Tyr Pro 
    130                 135                 140                 

Lys Thr Leu Phe Leu Leu Arg Gly Asn His Glu Cys Arg His Leu Thr 
145                 150                 155                 160 

Glu Tyr Phe Thr Phe Lys Gln Glu Cys Lys Ile Lys Tyr Ser Glu Arg 
                165                 170                 175     

Val Tyr Asp Ala Cys Met Asp Ala Phe Asp Cys Leu Pro Leu Ala Ala 
            180                 185                 190         

Leu Met Asn Gln Gln Phe Leu Cys Val His Gly Gly Leu Ser Pro Glu 
        195                 200                 205             

Ile Asn Thr Leu Asp Asp Ile Arg Lys Leu Asp Arg Phe Lys Glu Pro 
    210                 215                 220                 

Pro Ala Tyr Gly Pro Met Cys Asp Ile Leu Trp Ser Asp Pro Leu Glu 
225                 230                 235                 240 

Asp Phe Gly Asn Glu Lys Thr Gln Glu His Phe Thr His Asn Thr Val 
                245                 250                 255     

Arg Gly Cys Ser Tyr Phe Tyr Ser Tyr Pro Ala Val Cys Glu Phe Leu 
            260                 265                 270         

Gln His Asn Asn Leu Leu Ser Ile Leu Arg Ala His Glu Ala Gln Asp 
        275                 280                 285             

Ala Gly Tyr Arg Met Tyr Arg Lys Ser Gln Thr Thr Gly Phe Pro Ser 
    290                 295                 300                 

Leu Ile Thr Ile Phe Ser Ala Pro Asn Tyr Leu Asp Val Tyr Asn Asn 
305                 310                 315                 320 

Lys Ala Ala Val Leu Lys Tyr Glu Asn Asn Val Met Asn Ile Arg Gln 
                325                 330                 335     

Phe Asn Cys Ser Pro His Pro Tyr Trp Leu Pro Asn Phe Met Asp Val 
            340                 345                 350         

Phe Thr Trp Ser Leu Pro Phe Val Gly Glu Lys Val Thr Glu Met Leu 
        355                 360                 365             

Val Asn Val Leu Asn Ile Cys Ser Asp Asp Glu Leu Gly Ser Glu Glu 
    370                 375                 380                 

Asp 
385 


<210>  83
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single component system - FK506 sensor - FKBP

<400>  83

Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      

Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          

Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              

Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  

Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
65                  70                  75                  80  

Ala Tyr  Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala Thr 
                85                  90                  95      

Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105         

<210>  84
<211>  1392
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single component system - Cyclosporine A sensor - calcineurin 
       alpha/beta-GDH-CaM-cyclophilin

<400>  84

Asp His His His His His His Ser Ser Gly Thr Ser Glu Pro Lys Ala 
1               5                   10                  15      

Ile Asp Pro Lys Leu Ser Thr Thr Asp Arg Val Val Lys Ala Val Pro 
            20                  25                  30          

Phe Pro Pro Ser His Arg Leu Thr Ala Lys Glu Val Phe Asp Asn Asp 
        35                  40                  45              

Gly Lys Pro Arg Val Asp Ile Leu Lys Ala His Leu Met Lys Glu Gly 
    50                  55                  60                  

Arg Leu Glu Glu Ser Val Ala Leu Arg Ile Ile Thr Glu Gly Ala Ser 
65                  70                  75                  80  

Ile Leu Arg Gln Glu Lys Asn Leu Leu Asp Ile Asp Ala Pro Val Thr 
                85                  90                  95      

Val Cys Gly Asp Ile His Gly Gln Phe Phe Asp Leu Met Lys Leu Phe 
            100                 105                 110         

Glu Val Gly Gly Ser Pro Ala Asn Thr Arg Tyr Leu Phe Leu Gly Asp 
        115                 120                 125             

Tyr Val Asp Arg Gly Tyr Phe Ser Ile Glu Cys Val Leu Tyr Leu Trp 
    130                 135                 140                 

Ala Leu Lys Ile Leu Tyr Pro Lys Thr Leu Phe Leu Leu Arg Gly Asn 
145                 150                 155                 160 

His Glu Cys Arg His Leu Thr Glu Tyr Phe Thr Phe Lys Gln Glu Cys 
                165                 170                 175     

Lys Ile Lys Tyr Ser Glu Arg Val Tyr Asp Ala Cys Met Asp Ala Phe 
            180                 185                 190         

Asp Cys Leu Pro Leu Ala Ala Leu Met Asn Gln Gln Phe Leu Cys Val 
        195                 200                 205             

His Gly Gly Leu Ser Pro Glu Ile Asn Thr Leu Asp Asp Ile Arg Lys 
    210                 215                 220                 

Leu Asp Arg Phe Lys Glu Pro Pro Ala Tyr Gly Pro Met Cys Asp Ile 
225                 230                 235                 240 

Leu Trp Ser Asp Pro Leu Glu Asp Phe Gly Asn Glu Lys Thr Gln Glu 
                245                 250                 255     

His Phe Thr His Asn Thr Val Arg Gly Cys Ser Tyr Phe Tyr Ser Tyr 
            260                 265                 270         

Pro Ala Val Cys Glu Phe Leu Gln His Asn Asn Leu Leu Ser Ile Leu 
        275                 280                 285             

Arg Ala His Glu Ala Gln Asp Ala Gly Tyr Arg Met Tyr Arg Lys Ser 
    290                 295                 300                 

Gln Thr Thr Gly Phe Pro Ser Leu Ile Thr Ile Phe Ser Ala Pro Asn 
305                 310                 315                 320 

Tyr Leu Asp Val Tyr Asn Asn Lys Ala Ala Val Leu Lys Tyr Glu Asn 
                325                 330                 335     

Asn Val Met Asn Ile Arg Gln Phe Asn Cys Ser Pro His Pro Tyr Trp 
            340                 345                 350         

Leu Pro Asn Phe Met Asp Val Phe Thr Trp Ser Leu Pro Phe Val Gly 
        355                 360                 365             

Glu Lys Val Thr Glu Met Leu Val Asn Val Leu Asn Ile Cys Ser Asp 
    370                 375                 380                 

Asp Glu Leu Gly Ser Glu Glu Asp Gly Ser Gly Ser Gly Ser Gly Gly 
385                 390                 395                 400 

Gly Asn Glu Ala Ser Tyr Pro Leu Glu Met Cys Ser His Phe Asp Ala 
                405                 410                 415     

Asp Glu Ile Lys Arg Leu Gly Lys Arg Phe Lys Lys Leu Asp Leu Asp 
            420                 425                 430         

Asn Ser Gly Ser Leu Ser Val Glu Glu Phe Met Ser Leu Pro Glu Leu 
        435                 440                 445             

Gln Gln Asn Pro Leu Val Gln Arg Val Ile Asp Ile Phe Asp Thr Asp 
    450                 455                 460                 

Gly Asn Gly Glu Val Asp Phe Lys Glu Phe Ile Glu Gly Val Ser Gln 
465                 470                 475                 480 

Phe Ser Val Lys Gly Asp Lys Glu Gln Lys Leu Arg Phe Ala Phe Arg 
                485                 490                 495     

Ile Tyr Asp Met Asp Lys Asp Gly Tyr Ile Ser Asn Gly Glu Leu Phe 
            500                 505                 510         

Gln Val Leu Lys Met Met Val Gly Asn Asn Leu Lys Asp Thr Gln Leu 
        515                 520                 525             

Gln Gln Ile Val Asp Lys Thr Ile Ile Asn Ala Asp Lys Asp Gly Asp 
    530                 535                 540                 

Gly Arg Ile Ser Phe Glu Glu Phe Cys Ala Val Val Gly Gly Leu Asp 
545                 550                 555                 560 

Ile His Lys Lys Met Val Val Asp Val Gly Gly Ser Gly Ser Gly Ser 
                565                 570                 575     

Gly Gly Ser Gly Gly Gly Ser Gly Ser Gly Gly Gln Lys Ile Tyr Tyr 
            580                 585                 590         

Thr Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala Tyr Leu Phe Leu Pro 
        595                 600                 605             

Asn Gln Ala Gln His Thr Pro Thr Gln Gln Glu Leu Asn Gly Lys Asp 
    610                 615                 620                 

Tyr His Thr Tyr Met Gly Lys Val Leu Arg Leu Asn Leu Asp Gly Ser 
625                 630                 635                 640 

Ile Pro Lys Asp Asn Pro Ser Phe Asn Gly Val Val Ser His Ile Tyr 
                645                 650                 655     

Thr Leu Gly His Arg Asn Pro Gln Gly Leu Ala Phe Thr Pro Asn Gly 
            660                 665                 670         

Lys Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser Asp Asp Glu Ile Asn 
        675                 680                 685             

Leu Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro Asn Val Ala Gly Tyr 
    690                 695                 700                 

Lys Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr Ser Ala Ala Ala Asn 
705                 710                 715                 720 

Lys Thr Ile Lys Asp Leu Ala Gln Asn Gly Val Lys Val Ala Ala Gly 
                725                 730                 735     

Val Pro Val Thr Lys Glu Ser Glu Trp Thr Gly Lys Asn Phe Val Pro 
            740                 745                 750         

Pro Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr Tyr Asn Tyr Asn Asp 
        755                 760                 765             

Pro Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp Pro Thr Val Ala Pro 
    770                 775                 780                 

Ser Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys Ala Ile Thr Gly Trp 
785                 790                 795                 800 

Glu Asn Thr Leu Leu Val Pro Ser Leu Lys Arg Gly Val Ile Phe Arg 
                805                 810                 815     

Ile Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr Asp Asp Ala Val Pro 
            820                 825                 830         

Met Phe Lys Ser Gly Ser Gly Gly Thr Glu Glu Gln Ile Ala Glu Phe 
        835                 840                 845             

Lys Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr 
    850                 855                 860                 

Thr Lys Glu Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr 
865                 870                 875                 880 

Glu Ala Glu Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn 
                885                 890                 895     

Gly Thr Ile Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met 
            900                 905                 910         

Lys Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe 
        915                 920                 925             

Asp Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala Glu Leu Arg His Val 
    930                 935                 940                 

Met Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met 
945                 950                 955                 960 

Ile Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu 
                965                 970                 975     

Phe Val Gln Met Met Thr Ala Gly Gly Ser Gly Gly Asn Arg Tyr Arg 
            980                 985                 990         

Asp Val Ile Ala Ser Pro Asp Gly  Asn Val Leu Tyr Val  Leu Thr Asp 
        995                 1000                 1005             

Thr Ala  Gly Asn Val Gln Lys  Asp Asp Gly Ser Val  Thr Asn Thr 
    1010                 1015                 1020             

Leu Glu  Asn Pro Gly Ser Leu  Ile Lys Phe Thr Tyr  Lys Ala Lys 
    1025                 1030                 1035             

Gly Gly  Ser Gly Ser Gly Ser  Gly Ser Gly Gly Ser  Gly Ser Gly 
    1040                 1045                 1050             

Ser Gly  Gly Asp Val Pro Leu  Ile Pro Ser Gln Phe  Ala Lys Ala 
    1055                 1060                 1065             

Lys Ser  Glu Asn Phe Asp Lys  Lys Val Ile Leu Ser  Asn Leu Asn 
    1070                 1075                 1080             

Lys Pro  His Ala Leu Leu Trp  Gly Pro Asp Asn Gln  Ile Trp Leu 
    1085                 1090                 1095             

Thr Glu  Arg Ala Thr Gly Lys  Ile Leu Arg Val Asn  Pro Glu Ser 
    1100                 1105                 1110             

Gly Ser  Val Lys Thr Val Phe  Gln Val Pro Glu Ile  Val Asn Asp 
    1115                 1120                 1125             

Ala Asp  Gly Gln Asn Gly Leu  Leu Gly Phe Ala Phe  His Pro Asp 
    1130                 1135                 1140             

Phe Lys  Asn Asn Pro Tyr Ile  Tyr Ile Ser Gly Thr  Phe Lys Asn 
    1145                 1150                 1155             

Pro Lys  Ser Thr Asp Lys Glu  Leu Pro Asn Gln Thr  Ile Ile Arg 
    1160                 1165                 1170             

Arg Tyr  Thr Tyr Asn Lys Ser  Thr Asp Thr Leu Glu  Lys Pro Val 
    1175                 1180                 1185             

Asp Leu  Leu Ala Gly Leu Pro  Ser Ser Lys Asp His  Gln Ser Gly 
    1190                 1195                 1200             

Arg Leu  Val Ile Gly Pro Gly  Gly Ser Gly Ser Gly  Ser Gly Gly 
    1205                 1210                 1215             

Ser Gly  Gly Ser Gly Ser Gly  Gly Gly Met Val Asn  Pro Thr Val 
    1220                 1225                 1230             

Phe Phe  Asp Ile Ala Val Asp  Gly Glu Pro Leu Gly  Arg Val Ser 
    1235                 1240                 1245   

Phe Glu  Leu Phe Ala Asp Lys  Val Pro Lys Thr Ala  Glu Asn Phe 
    1250                 1255                 1260             

Arg Ala  Leu Ser Thr Gly Glu  Lys Gly Phe Gly Tyr  Lys Gly Ser 
    1265                 1270                 1275             

Cys Phe  His Arg Ile Ile Pro  Gly Phe Met Cys Gln  Gly Gly Asp 
    1280                 1285                 1290             

Phe Thr  Arg His Asn Gly Thr  Gly Gly Lys Ser Ile  Tyr Gly Glu 
    1295                 1300                 1305             

Lys Phe  Glu Asp Glu Asn Phe  Ile Leu Lys His Thr  Gly Pro Gly 
    1310                 1315                 1320             

Ile Leu  Ser Met Ala Asn Ala  Gly Pro Asn Thr Asn  Gly Ser Gln 
    1325                 1330                 1335             

Phe Phe  Ile Cys Thr Ala Lys  Thr Glu Trp Leu Asp  Gly Lys His 
    1340                 1345                 1350             

Val Val  Phe Gly Lys Val Lys  Glu Gly Met Asn Ile  Val Glu Ala 
    1355                 1360                 1365             

Met Glu  Arg Phe Gly Ser Arg  Asn Gly Lys Thr Ser  Lys Lys Ile 
    1370                 1375                 1380             

Thr Ile  Ala Asp Cys Gly Gln  Leu Glu 
    1385                 1390         


<210>  85
<211>  846
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single component system - Amylase sensor - VHH1-GDH-CaM-VHH2

<400>  85

Asp Thr Thr Val Ser Glu Pro Ala Pro Ser Cys Val Thr Leu Tyr Gln 
1               5                   10                  15      

Ser Trp Arg Tyr Ser Gln Ala Asp Asn Gly Cys Ala Glu Thr Val Thr 
            20                  25                  30          

Val Lys Val Val Tyr Glu Asp Asp Thr Glu Gly Leu Cys Tyr Ala Val 
        35                  40                  45              

Ala Pro Gly Gln Ile Thr Thr Val Gly Asp Gly Tyr Ile Gly Ser His 
    50                  55                  60                  

Gly His Ala Arg Tyr Leu Ala Arg Cys Leu Gly Gly Ser Gly Gly Ser 
65                  70                  75                  80  

Gly Gly Gln Lys Ile Tyr Tyr Thr Ile Gly Asp Gln Gly Arg Asn Gln 
                85                  90                  95      

Leu Ala Tyr Leu Phe Leu Pro Asn Gln Ala Gln His Thr Pro Thr Gln 
            100                 105                 110         

Glu Glu Leu Asn Gly Lys Asp Tyr His Thr Tyr Met Gly Lys Val Leu 
        115                 120                 125             

Arg Leu Asn Leu Asp Gly Ser Ile Pro Lys Asp Asn Pro Ser Phe Asn 
    130                 135                 140                 

Gly Val Val Ser His Ile Tyr Thr Leu Gly His Arg Asn Pro Gln Gly 
145                 150                 155                 160 

Leu Ala Phe Thr Pro Asn Gly Lys Leu Leu Gln Ser Glu Gln Gly Pro 
                165                 170                 175     

Asn Ser Asp Asp Glu Ile Asn Leu Ile Val Lys Gly Gly Asn Tyr Gly 
            180                 185                 190         

Trp Pro Asn Val Ala Gly Tyr Lys Asp Asp Ser Gly Tyr Ala Tyr Ala 
        195                 200                 205             

Asn Tyr Ser Ala Ala Ala Asn Lys Thr Ile Lys Asp Leu Ala Gln Asn 
    210                 215                 220                 

Gly Val Lys Val Ala Ala Gly Val Pro Val Thr Lys Glu Ser Glu Trp 
225                 230                 235                 240 

Thr Gly Lys Asn Phe Val Pro Pro Leu Lys Thr Leu Tyr Thr Val Gln 
                245                 250                 255     

Asp Thr Tyr Asn Tyr Asn Asp Pro Thr Cys Gly Glu Met Thr Tyr Ile 
            260                 265                 270         

Cys Trp Pro Thr Val Ala Pro Ser Ser Ala Tyr Val Tyr Lys Gly Gly 
        275                 280                 285

Lys Lys Ala Ile Thr Gly Trp Glu Asn Thr Leu Leu Val Pro Ser Leu 
    290                 295                 300                 

Lys Arg Gly Val Ile Phe Arg Ile Lys Leu Asp Pro Thr Tyr Ser Thr 
305                 310                 315                 320 

Thr Tyr Asp Asp Ala Val Pro Met Phe Lys Ser Gly Ser Gly Gly Thr 
                325                 330                 335     

Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp Lys 
            340                 345                 350         

Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr Val Met Arg 
        355                 360                 365             

Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp Met Ile Asn 
    370                 375                 380                 

Glu Val Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe Leu 
385                 390                 395                 400 

Thr Met Met Ala Arg Lys Met Lys Asp Thr Asp Ser Glu Glu Glu Ile 
                405                 410                 415     

Arg Glu Ala Phe Arg Val Phe Asp Lys Asp Gly Asn Gly Tyr Ile Ser 
            420                 425                 430         

Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu Lys Leu Thr 
        435                 440                 445             

Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile Asp Gly Asp 
    450                 455                 460                 

Gly Gln Val Asn Tyr Glu Glu Phe Val Gln Met Met Thr Ala Gly Gly 
465                 470                 475                 480 

Ser Gly Gly Asn Arg Tyr Arg Asp Val Ile Ala Ser Pro Asp Gly Asn 
                485                 490                 495     

Val Leu Tyr Val Leu Thr Asp Thr Ala Gly Asn Val Gln Lys Asp Asp 
            500                 505                 510         

Gly Ser Val Thr Asn Thr Leu Glu Asn Pro Gly Ser Leu Ile Lys Phe 
        515                 520                 525   
          
Thr Tyr Lys Ala Lys Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Gly 
    530                 535                 540                 

Ser Gly Ser Gly Ser Gly Gly Asp Val Pro Leu Ile Pro Ser Gln Phe 
545                 550                 555                 560 

Ala Lys Ala Lys Ser Glu Asn Phe Asp Lys Lys Val Ile Leu Ser Asn 
                565                 570                 575     

Leu Asn Lys Pro His Ala Leu Leu Trp Gly Pro Asp Asn Gln Ile Trp 
            580                 585                 590         

Leu Thr Glu Arg Ala Thr Gly Lys Ile Leu Arg Val Asn Pro Glu Ser 
        595                 600                 605             

Gly Ser Val Lys Thr Val Phe Gln Val Pro Glu Ile Val Asn Asp Ala 
    610                 615                 620                 

Asp Gly Gln Asn Gly Leu Leu Gly Phe Ala Phe His Pro Asp Phe Lys 
625                 630                 635                 640 

Asn Asn Pro Tyr Ile Tyr Ile Ser Gly Thr Phe Lys Asn Pro Lys Ser 
                645                 650                 655     

Thr Asp Lys Glu Leu Pro Asn Gln Thr Ile Ile Arg Arg Tyr Thr Tyr 
            660                 665                 670         

Asn Lys Ser Thr Asp Thr Leu Glu Lys Pro Val Asp Leu Leu Ala Gly 
        675                 680                 685             

Leu Pro Ser Ser Lys Asp His Gln Ser Gly Arg Leu Val Ile Gly Pro 
    690                 695                 700                 

Gly Ser Gly Ser Gly Ser Gly Gly Gln Val Gln Leu Val Glu Ser Gly 
705                 710                 715                 720 

Gly Gly Thr Val Pro Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala 
                725                 730                 735     

Ser Gly Asn Thr Leu Cys Thr Tyr Asp Met Ser Trp Tyr Arg Arg Ala 
            740                 745                 750         

Pro Gly Lys Gly Arg Asp Phe Val Ser Gly Ile Asp Asn Asp Gly Thr 
        755                 760                 765             

Thr Thr Tyr Val Asp Ser Val Ala Gly Arg Phe Thr Ile Ser Gln Gly 
    770                 775                 780                 

Asn Ala Lys Asn Thr Ala Tyr Leu Gln Met Asp Ser Leu Lys Pro Asp 
785                 790                 795                 800 

Asp Thr Ala Met Tyr Tyr Cys Lys Pro Ser Leu Arg Tyr Gly Leu Pro 
                805                 810                 815     

Gly Cys Pro Ile Ile Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser 
            820                 825                 830         

Ser Lys Leu Ala Ala Ala Leu Glu His His His His His His 
        835                 840                 845     


<210>  86
<211>  622
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Single component system - cpGDH-CaM

<400>  86

Gln Lys Ile Tyr Tyr Thr Ile Gly Asp Gln Gly Arg Asn Gln Leu Ala 
1               5                   10                  15      

Tyr Leu Phe Leu Pro Asn Gln Ala Gln His Thr Pro Thr Gln Gln Glu 
            20                  25                  30          

Leu Asn Gly Lys Asp Tyr His Thr Tyr Met Gly Lys Val Leu Arg Leu 
        35                  40                  45              

Asn Leu Asp Gly Ser Ile Pro Lys Asp Asn Pro Ser Phe Asn Gly Val 
    50                  55                  60                  

Val Ser His Ile Tyr Thr Leu Gly His Arg Asn Pro Gln Gly Leu Ala 
65                  70                  75                  80  

Phe Thr Pro Asn Gly Lys Leu Leu Gln Ser Glu Gln Gly Pro Asn Ser 
                85                  90                  95      

Asp Asp Glu Ile Asn Leu Ile Val Lys Gly Gly Asn Tyr Gly Trp Pro 
            100                 105                 110         

Asn Val Ala Gly Tyr Lys Asp Asp Ser Gly Tyr Ala Tyr Ala Asn Tyr 
        115                 120                 125             

Ser Ala Ala Ala Asn Lys Thr Ile Lys Asp Leu Ala Gln Asn Gly Val 
    130                 135                 140                 

Lys Val Ala Ala Gly Val Pro Val Thr Lys Glu Ser Glu Trp Thr Gly 
145                 150                 155                 160 

Lys Asn Phe Val Pro Pro Leu Lys Thr Leu Tyr Thr Val Gln Asp Thr 
                165                 170                 175     

Tyr Asn Tyr Asn Asp Pro Thr Cys Gly Glu Met Thr Tyr Ile Cys Trp 
            180                 185                 190         

Pro Thr Val Ala Pro Ser Ser Ala Tyr Val Tyr Lys Gly Gly Lys Lys 
        195                 200                 205             

Ala Ile Thr Gly Trp Glu Asn Thr Leu Leu Val Pro Ser Leu Lys Arg 
    210                 215                 220                 

Gly Val Ile Phe Arg Ile Lys Leu Asp Pro Thr Tyr Ser Thr Thr Tyr 
225                 230                 235                 240 

Asp Asp Ala Val Pro Met Phe Lys Ser Gly Ser Gly Gly Thr Glu Glu 
                245                 250                 255     

Gln Ile Ala Glu Phe Lys Glu Ala Phe Ser Leu Phe Asp Lys Asp Gly 
            260                 265                 270         

Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr Val Met Arg Ser Leu 
        275                 280                 285             

Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp Met Ile Asn Glu Val 
    290                 295                 300                 

Asp Ala Asp Gly Asn Gly Thr Ile Asp Phe Pro Glu Phe Leu Thr Met 
305                 310                 315                 320 

Met Ala Arg Lys Met Lys Asp Thr Asp Ser Glu Glu Glu Ile Arg Glu 
                325                 330                 335     

Ala Phe Arg Val Phe Asp Lys Asp Gly Asn Gly Tyr Ile Ser Ala Ala 
            340                 345                 350         

Glu Leu Arg His Val Met Thr Asn Leu Gly Glu Lys Leu Thr Asp Glu 
        355                 360                 365             

Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile Asp Gly Asp Gly Gln 
    370                 375                 380                 

Val Asn Tyr Glu Glu Phe Val Gln Met Met Thr Ala Gly Gly Ser Gly 
385                 390                 395                 400 

Gly Asn Arg Tyr Arg Asp Val Ile Ala Ser Pro Asp Gly Asn Val Leu 
                405                 410                 415     

Tyr Val Leu Thr Asp Thr Ala Gly Asn Val Gln Lys Asp Asp Gly Ser 
            420                 425                 430         

Val Thr Asn Thr Leu Glu Asn Pro Gly Ser Leu Ile Lys Phe Thr Tyr 
        435                 440                 445             

Lys Ala Lys Gly Gly Ser Gly Ser Gly Ser Gly Ser Gly Gly Ser Gly 
    450                 455                 460                 

Ser Gly Ser Gly Gly Asp Val Pro Leu Ile Pro Ser Gln Phe Ala Lys 
465                 470                 475                 480 

Ala Lys Ser Glu Asn Phe Asp Lys Lys Val Ile Leu Ser Asn Leu Asn 
                485                 490                 495     

Lys Pro His Ala Leu Leu Trp Gly Pro Asp Asn Gln Ile Trp Leu Thr 
            500                 505                 510         

Glu Arg Ala Thr Gly Lys Ile Leu Arg Val Asn Pro Glu Ser Gly Ser 
        515                 520                 525             

Val Lys Thr Val Phe Gln Val Pro Glu Ile Val Asn Asp Ala Asp Gly 
    530                 535                 540                 

Gln Asn Gly Leu Leu Gly Phe Ala Phe His Pro Asp Phe Lys Asn Asn 
545                 550                 555                 560 

Pro Tyr Ile Tyr Ile Ser Gly Thr Phe Lys Asn Pro Lys Ser Thr Asp 
                565                 570                 575     

Lys Glu Leu Pro Asn Gln Thr Ile Ile Arg Arg Tyr Thr Tyr Asn Lys 
            580                 585                 590         

Ser Thr Asp Thr Leu Glu Lys Pro Val Asp Leu Leu Ala Gly Leu Pro 
        595                 600                 605             

Ser Ser Lys Asp His Gln Ser Gly Arg Leu Val Ile Gly Pro 
    610                 615                 620         

